徽州贡菊中熊果酸的高压脉冲电场技术(HIPEF)辅助提取及纯化工艺研究  

High intensity pulsed electric fields( HIPEF)- assisted extraction and purification of ursolic acid from Huizhou Gongju

作　　者：孙克奎[1] 金声琅[1] 王莹[2] 

机构地区：[1]黄山学院旅游学院,安徽黄山245021 [2]青岛农业大学食品科学与工程学院,山东青岛266109

出　　处：《食品工业科技》2015年第1期207-210,共4页Science and Technology of Food Industry

基　　金：2014年度安徽省教育厅自然科学研究重点项目(KJ2014A243);2013年度安徽省高校重点研究基地招标项目(SK2013B484)

摘　　要：本文以徽州贡菊为原料,以熊果酸得率为指标,利用高压脉冲电场技术(HIPEF)辅助提取徽州贡菊中熊果酸。最后确定的最佳提取参数是:电场强度30k V/cm、脉冲数2、料液比1∶12、温度30℃,最终熊果酸的提取率可达97.577%;并确定了使用X-5大孔树脂,上样量60BV、p H11、吸附速率为2BV/h、洗脱剂用70%乙醇、2BV/h的洗脱速度纯化效果最佳,最终徽州贡菊熊果酸的纯度可以达到85%以上。The effects of the HIPEF-assisted extraction of ursolic acid from Huizhou Gongju were preliminary investigated, in which the technical parameters were optimized.The optimal conditions were as follows：electric field intensity of 30 kV/cm, pulse number of 2, material- solvent ratio of 1 ： 12 and 30℃. Under these conditions, the extraction yield of ursolic acid can reach to 97.577%.The results showed that the X-5 macroporons resin had the best effect on the separation when the volume of drug 60BV with the adsorption-speed 2BV/h, pH11 ,and the volume of 70% ethanol with desorption-speed 2BV/h,and the purity of ursolic acid could reach to 85%.

关 键 词：徽州贡菊 熊果酸 HIPEF辅助提取 纯化 

分 类 号：TS255.5[轻工技术与工程—农产品加工及贮藏工程]