巨菌草纤维素的酶解条件  

Enzymolysis conditions of Pennisetumsp.cellulose

作　　者：师静[1,2] 林占熺[1,2] 林冬梅 苏德伟[1,2] 罗海凌 林兴生 林占森 郑丹[1,2] 陈锦华[1,2] 姚俊新 

机构地区：[1]国家菌草工程技术研究中心,福建福州350002 [2]福建农林大学生命科学学院,福建福州350002

出　　处：《草业科学》2014年第4期760-765,共6页Pratacultural Science

基　　金：国家林业局公益性行业项目"生态脆弱区发展菌草业关键技术的研究"(201004019);福建省科技重大专项(2012NZ0002)

摘　　要：纤维素酶酶解植物纤维的过程受许多因素的影响。选取酶解时间、纤维素酶用量、底物浓度、缓冲液pH值和反应温度5个因素对巨菌草(Pennisetumsp.)纤维素酶解条件进行初步研究。结果表明,各因素对该试验的影响效果依次为纤维素酶用量>酶解时间>底物浓度>缓冲液pH值>反应温度。酶解巨菌草纤维素的最佳条件:反应时间60h,酶用量44.8U·g-1,底物浓度10g·L-1,pH值5.0,酶解温度45℃。在该条件下进行酶解反应,酶解液中还原糖含量为95.509mg·g-1。该研究为巨菌草作为制备生物乙醇的材料提供了理论依据。The present study analyzed the effects of five factors （enzymatic time, enzyme dosage, substrate concentration, buffer pH and reaction temperature） on enzymatic reaction of Penniseturn sp. cellulose. The results showed that the effects of these different factors were different and the activity of cellulose enzyme decreased as follows order： enzyme dosage）enzymolysis time〉 substrate concentration〉 buffer pH〉temperature. The optimal enzymolysis condition of Penniseturn sp. was 60 h reaction time, 44. 8 U · g 1 enzyme dosage, 10 g · 1- i substrate concentration, pH 5. 0 and 45 ℃ hydrolysis temperature. Under these optimal conditions, enzymatic hydrolyzate of sugar content was 102. 656 mg · g- 1. These results conducted a preliminary exploration for Pennisetum sp. as bio-ethanol fuel.

关 键 词：巨菌草 纤维素 酶解 条件优化 

分 类 号：TQ352.2[化学工程]