HPLC法测定草原老鹳草中莽草酸和没食子酸  

Determination of shikimic acid and gallic acid in Geranium pratense L. by HPLC

作　　者：谢松[1] 宋良科[1] 万军[1] 童志平[1] 谭睿[1] 刘小珍[1] 

机构地区：[1]西南交通大学生命科学与工程学院,四川成都610031

出　　处：《中成药》2014年第12期2554-2556,共3页Chinese Traditional Patent Medicine

基　　金：四川省藏药标准制定工作子项目(024131113010040)

摘　　要：目的利用HPLC法对草原老鹳草中莽草酸和没食子酸进行定量测定,建立草原老鹳草定量分析的质量控制标准。方法 Agilent TC-C18色谱柱（4.6 mm×250 mm,5μm）,以乙腈-1%磷酸溶液（5∶95）为流动相,体积流量为0.6 m L/min,检测波长为211 nm,柱温25℃。结果莽草酸、没食子酸的线性范围分别为0.196-1.372μg（r=0.999 5）、0.270-1.890μg（r=0.999 9）,平均回收率为99.7%（RSD 1.7%）、101.5%（RSD 1.6%）。结论 9批药材中莽草酸平均质量分数为1.937 9 mg/g,没食子酸平均质量分数为2.438 7 mg/g,可作为草原老鹳草药材的质量控制方法。AIM To establish an HPLC method for the determination of shikimic acid and gallic acid in Geranium pratense L. METHODS The analytical column was Agilent TC-C18column（ 4. 6 mm × 250 mm,5 μm）with acetonitrile-1% aqueous acetic acid（ 5 ∶ 95） as mobile phase. The flow rate of 0. 6 m L / min,the UV detection was set at 211 nm and the column temperature was at 25 ℃. RESULTS The linear ranges were 0. 196 ~1. 372 μg for shikimic acid（ r = 0. 999 5） and 0. 270 ~ 1. 890 μg for gallic acid（ r = 0. 999 9）,respectively.The average recoveries of shikimic acid and gallic acid were 99. 7%（ RSD 1. 7%） and 101. 5%（ RSD 1. 6%）,respectively. CONCLUSION The nine batches of herbs that the average content of shikimic acid is 1. 937 9mg / g and the average content of gallic acid is 2. 438 7 mg / g. The method could be used for quality control for this herb.

关 键 词：草原老鹳草 莽草酸 没食子酸 质量控制 高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]