Cr(VI)对脊尾白虾(Exopalaemon carinicauda)幼虾暴露和恢复期肝胰脏的SOD活性、MDA及MTs含量的影响  

Effects of Cr(VI) Exposure and Recovery on the SOD Activities,Contents of MDA and MTs in Hepatopancreas Tissue of Juvenile Exopalaemon carinicauda

作　　者：李磊[1] 蒋玫[1] 沈新强[1] 王云龙[1] 吴庆元[2] 牛俊翔[2] 许高鹏[2] 

机构地区：[1]中国水产科学研究院东海水产研究所,上海200090 [2]上海海洋大学海洋科学学院,上海201306

出　　处：《生态毒理学报》2014年第6期1226-1231,共6页Asian Journal of Ecotoxicology

基　　金：上海市科学技术委员会项目(12231203403);中国水产科学研究院基本科研业务费(2014A02XK01);中央级公益性科研院所基本科研业务费专项(2014T06)

摘　　要：为了探索水体中Cr(VI)对脊尾白虾(Exopalaemon carinicauda)的毒性效应,通过Cr(VI)的暴露(15 d)和清水恢复实验(15 d),研究了Cr(VI)对脊尾白虾肝胰脏的SOD活性、MDA及MTs含量的影响。结果表明,通过15 d的暴露,5 mg·L-1(高浓度)、0.5 mg·L-1(低浓度)实验组肝胰脏的SOD均显著高于对照组水平(p<0.05),15 d的清水恢复后,两个浓度实验组均能够恢复到对照组水平(p>0.05)。MDA在暴露阶段呈现持续上升的趋势,且在15 d后两个浓度实验组MDA的含量均显著高于对照组水平(p<0.05),15 d的清水恢复后,5 mg·L-1实验组的MDA含量依然显著高于对照组水平(p<0.05),0.5mg·L-1实验组的MDA含量基本恢复到对照组水平(p>0.05)。通过15 d的暴露,两个浓度实验组MTs的含量均显著高于对照组水平(p<0.05),15 d的清水恢复后,两个浓度实验组MTs的含量均能够恢复到对照组水平(p>0.05)。Abstract： In order to examine the effect of Cr（VI） exposure on of marine biota, the present study analyzedan-tioxidant enzyme （SOD）activities, contents of lipid peroxidation levels （LPO） and metallothioneins （MTs） of juvenile rigid-tail prawn Exopalaemon carinicauda in hepatopancreas tissue. The experimental juvenile prawn-swerereared at low （0.5 mg·L-1） and high Cr（VI） （5 mg·L-1） concentrations in the fast 15 d exposure experiment, respectively. Then, they were put in clean seawater for recovery in the next 15 d. The results show that SODactivities were significantly higher at two exposure groups than at the control group （p 〈0.05）. By the end of 15 d recovery period, SOD activities in two exposure treatments were similar to that at the control （p 〉 0.05）. The MDA concentration in hepatoPancreas tissue at the low and high concentration treatments showed an inereasingtrend during the exposure period, and the MDA concentration at two treatment groups were signifi cantly higher than that at the control group （p 〈0.05） at the end of 15 d exposure experiment. Through 15 d re- covery, MDA concentrations at low concentration treatments showed obviously decreasing and had no differ- ences with that at the control group（p 〉0.05）. MDA concentrations at the high concentration treatments were still significantly higher than that at the control groups （p 〈0.05）. The MTs concentrations at two treatment groups were significantly higher than that at the control group（p 〈0.05） at the end of 15 d exposure experiment, but MTs concentrations could recover to the value at the control group through 15 d recovery（p 〉0.05）.

关 键 词：脊尾白虾 六价铬 肝胰脏 毒理学指标 

分 类 号：X171.5[环境科学与工程—环境科学]