油炸小食品中乙二胺四乙酸二钠的高效液相色谱法测定  

Determination of Disodium EDTA in Fried Small Foods by Using HPLC Method

作　　者：陈肇臻 刘小刚 

机构地区：[1]龙岩市产品质量检验所,福建龙岩364000

出　　处：《质量技术监督研究》2014年第5期5-7,10,共4页Quality and Technical Supervision Research

摘　　要：目的文中建立一种测定油炸食品中乙二胺四乙酸二钠的液相色谱检测方法。方法油炸小食品经无水乙醚除油并挥去乙醚,水萃取,加入氯化铜溶液及抗坏血酸溶液形成Cu-EDTA络合物。色谱分离时使用C18分析柱,甲醇、四正丁基溴化铵缓冲液为流动相,梯度洗脱,245nm波长检测。结果该方法线性范围20.00μg·ml^-1~400.00μg·ml^-1,相关系数r：0.9999,检出限6.5mg·kg^-1,定量限21mg·kg^-1。回收率：93.7~100.7%;精密度：RSD60.2%。结论该方法的准确性和精密度满足检测要求需要。Objective：A method of determination of disodium EDTA of fried small foods by HPLC is established in this paper. Method： Through removing oil by anhydrous ether, evaporating ether subsequently, EDTA in fried small foods is then extracted by water. Addition of CuC12 solution and ascorbic acid solution, then the Cu-EDTA complex combond can be formed. In the chromatographic separation, C18 analysis column is served as isolated column, methanol mixed with tetra-n-butylammonium bromide buffer solution（pH=4.0）is used as mobile phase, gradient elution program is made and the detection wavelength is 245 nm. Result： Linear range of the standard curve is 20.00μg·ml^-1 400.00μg·ml^-1 with the correlation coefficient r=0.9999. The recovery rate of the method is 93.7 ~ 100.7%.The detection limit is 6.5mg·kg^-1 and the quantitative limit of the method is 21mg·kg^-1. Conclusion Precision and accuracy of this method meets the requirements of the detection.

关 键 词：油炸小食品 乙二胺四乙酸二钠 梯度洗脱 反相高效液相色谱法 

分 类 号：O657.72[理学—分析化学]