Lupeol对肝癌SMMC-7721细胞增殖及凋亡的影响  

Effect of lupeol on proliferation and apoptosis of hepatocellular carcinoma SMMC-7721 cells

作　　者：李悦[1] 薛平[1] 曹良启[1] 卢海武[1] 郑强[1] 温子龙[1] 张大伟[1] 

机构地区：[1]广州医科大学附属第二医院肝胆外科,广州510260

出　　处：《岭南现代临床外科》2014年第6期624-627,共4页Lingnan Modern Clinics in Surgery

基　　金：广东省科技计划项目(编号:2012B061700047);广州医科大学博士启动项目(编号:2012C08)

摘　　要：目的探讨羽扁豆醇(lupeol)对人肝癌SMMC-7721细胞生长和凋亡的作用及机制。方法采用MTT法检测lupeol对肝癌细胞SMMC-7721和正常肝细胞L-02的增殖抑制作用,流式细胞法检测细胞凋亡情况,Western blot检测TRAIL受体及抗凋亡蛋白表达变化,比色法分析caspase-8、-9、-3酶活性改变。结果 lupeol对SMMC-7721细胞具有明显的增殖抑制作用(IC50=40μmol/L),而对正常肝细胞L-02无细胞毒作用;lupeol在30、40、50μmol/L浓度范围诱导SMMC-7721细胞48小时的凋亡率分别为5.5%、12.6%、28%;抗凋亡蛋白c-FLIPL表达下调,caspase-8及caspase-3活性增强均呈剂量依赖性。结论 Lupeol通过下调c-FLIPL表达激活caspase通路诱导肝癌细胞凋亡,对肝癌细胞增殖发挥抑制作用。Objective To explore the effect and mechanism of lupeol on proliferation and apoptosis of hepatocellular carcinoma SMMC-7721 cells. Methods The effect of lupeol on growth inhibition of hepatocellular carcinoma SMMC-7721 cells and normal liver cells L-02 were analyzed by MTT assay. Cell apoptosis were measured and analyzed by flow cytometry and caspase enzymatic assays, and the expressions of TRAIL receptor and anti-apoptotic proteins were determined by Western blot. Results The proliferation of SMMC-7721 cells were dramatically inhibited by lupeol（IC50=40 μmol / L）, with almost no toxicity to normal cells L-02. The apoptotic rates of SMMC-7721 cells were 5.5%, 12.6%,and 28% treated with lupeol for 48 hours at concentration distribution 30, 40, and 50 μmol / L. The expressions of anti-apoptotic pretein c-FLIPLwas down-regulated and the activities of caspase-8 and-3were increased in a concentration-dependent manner. Conclusion Lupeol induces apoptosis of hepatocellular carcinoma cells through down-regulation of c-FLIPLand activation of caspase-dependent pathways, consequently inhibiting proliferation of cells.

关 键 词：羽扁豆醇 肝癌细胞 凋亡 c-FLIPL 

分 类 号：R735.7[医药卫生—肿瘤]