RP-HPLC同时测定杨树花和杨树叶中3个植物甾醇含量  

Determination of three phytosterols in Flos Populi Canadensis and leaves of Populus canadensis Moench by RP-HPLC

作　　者：侯莹莹[1] 赵岩[1] 唐国胜[1] 蔡恩博[1] 王士杰[2] 张连学[1] 

机构地区：[1]吉林农业大学中药材学院,长春130118 [2]吉林农业科技学院中药学院,吉林132101

出　　处：《药物分析杂志》2014年第12期2150-2153,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：国家科技支撑计划项目(2011BAI03B01);国家公益性行业科研专项(201303111);吉林省科技发展计划项目(20126046;20130303094;2014020413YY;YYZX201258;20140311050YY);吉林省产业技术体系项目(201218);吉林省中医药产业发展专项(YYZX201258;2009GJB10031);国家自然科学基金(31000154)

摘　　要：建立一种同时测定杨树花和杨树叶中豆甾醇、麦角甾醇、β-谷甾醇含量的反相高效液相色谱法。方法：以甲醇为流动相,测定杨树花和杨树叶中豆甾醇、麦角甾醇、β-谷甾醇的含量。结果：杨树花和杨树叶中3个植物甾醇的线性范围为0.00008-0.08μmol·m L-1,r〉0.9990。杨树花中麦角甾醇、豆甾醇、β-谷甾醇的加样回收率分别在92.6%-100.0%、93.9%-100.1%、94.0%-100.0%之间,RSD（n=6）分别为2.8%、2.2%、2.8%;杨树叶中各甾醇加样回收率分别在91.3%-100.0%、91.2%-100.0%、92.5%-100.1%之间,RSD（n=6）分别为2.7%、2.7%、2.5%。结论：该方法稳定、快速,精密度高,线性范围宽,适合杨树花和杨树叶中的豆甾醇、麦角甾醇、β-谷甾醇含量测定。Objective： To establish a method for determination of the contents of three phytosterols（stigmasterol, ergosterol,β- sitosterol）from Flos Populi Canadensis and leaves of Populus canadensis Moench by RP- HPLC. Methods： With methanol as the mobile phase,HPLC method was adopted to determine contents of phytosterols in Flos Populi Canadensis and leaves of Populus canadensis Moench. Results： The three phytosterols had good linearity in their ranges of 0. 00008 - 0. 08 μmol · mL- 1 （ the coefficients of correlation were all over 0. 9990）. The average recoveries of stigmasterol ,ergosterol β- sitosterol in Flos Populi Canadensis were 92.6% - 100.0% ,93.9% - 100. 1% ,94.0% - 100. 0% ,and RSDs（n =6）were 2. 8% ,2.2% ,2. 8% ,respectively;the av- erage recoveries（ n = 6）of the three phytosterols in leaves of Populus canadensis Moench were 91.3% - 100.0% , 91.2% - 100. 0% ,92.5% - 100. 1% ,and RSDs were 2.7% ,2.7% ,2.5% ,respectively. Conclusion ： The method is sensitive, accurate and reproducible, and reliable for the determination of phytosterols in Flos Populi Canadensis and leaves of Populus canadensis Moench.

关 键 词：杨树花 杨树叶 植物甾醇 豆甾醇 麦角甾醇 谷甾醇 含量测定 反相高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]