龋病患者龈上菌斑微生物多样性的研究  

Analysis of microbial diversity of supra-gingival plaque in dental caries

作　　者：吴维悦 侯本祥[1] 

机构地区：[1]首都医科大学口腔医学院牙体牙髓科,100050

出　　处：《中华口腔医学杂志》2014年第12期742-747,共6页Chinese Journal of Stomatology

基　　金：国家科技支撑计划（2012BAI07B03）;首都卫生发展科研专项（首发2011-2014-05）

摘　　要：目的 探讨有龋和无龋成年人龈上菌斑中微生物的组成特点及多样性差异,以期为龋病的病因学研究提供依据.方法 有龋组选择2013年7月至9月于首都医科大学口腔医学院牙体牙髓科就诊的患者,男女各8例,年龄18～ 35岁,龋失补牙数（decayed tooth and decayedmissing-filled-tooth,DMFT） ≥6且龋齿数≥3;无龋组选择同年龄段DMFT=0的知情同意者,男女各8名.每组16例.分别采集两组受试者龈上菌斑样本,应用PCR-变性梯度凝胶电泳（PCR-denaturing gradient gel electrophoresis,PCR-DGGE）联合克隆测序法对细菌组成进行研究.结果 两组共检测到6个菌门、28个菌属、88个菌种.有龋组中普氏菌属、二氧化碳噬纤维菌属、放线菌属、韦荣菌属及棒状杆菌属的检出率较高,这5个菌属克隆数的总和占有龋组总克隆数的56.2％（334/594）.与有龋组相比,无龋组拥有更丰富的优势菌属组合,普氏菌属、韦荣菌属、二氧化碳噬纤维菌属、棒状杆菌属、链球菌属、放线菌属、Aggregatibacter菌属和奈瑟菌属的检出率较高,8个菌属的克隆总数占无龋组总克隆数的65.2％（354/543）.有龋组微生物在菌种分类水平上的各个多样性指数均显著低于无龋组,差异有统计学意义 （P＜0.05）.结论 龋病的发生并非由单一菌种所致,可能为多种细菌共同作用的结果;随着龋病的发展,龈上菌斑中的微生物多样性下降.Objective To investigate the microbial composition and differences in dental plaque of caries and caries-free adults.Methods Caries-active（n=16） was defined as an individual who had at least three untreated decayed tooth and decayed-missing-filled-tooth（DMFT） score greater than 6.Caries-free （n=16） was defined as an individual who had a DMFT score equal to zero.The patients were 18-35 years old.Samples from supra-gingival plaque were obtained and PCR-denaturing gel electrophoresis cloning and sequencing of caries pathogens were used to catch the core microbial of dental caries.Results Six phylum,28 genus and 88 species were detected.In caries group,Prevotella,Capnocytophaga,Actinomyces,Veillonella and Corynebacterium were predominant,accounting for 56.2% （334/594） of the total cloning number of caries group.Caries-free group contained more predonminant genus than caries group.Prevotella,Veillonella,Capnocytophaga,Corynebacterium,Streptococcus,Actinomyces,Aggregatibacter and Neisseria were predominant,accounting for 65.2% （354/543） of the total cloning number of caries-free group.Caries group had less diversity than the caries-free group.The difference was statistically significant（P〈0.05）.Conclusions Caries might be caused by potentially pathogenic microbial communities rather than a single pathogen.In the progress of dental caries,the microbial diversity decreased.

关 键 词：菌斑微生物 16SrDNA PCR-DGGE 克隆测序 

分 类 号：R781.1[医药卫生—口腔医学]