牙本质内源性蛋白聚糖对全酸蚀粘接剂即刻粘接性能的影响  

Effect of dentin proteoglycans on the bonding properties of dentin

作　　者：高宇[1] 赵三军[2] 王培欢[3] 芦帅[2] 李晓静[2] 陈吉华[2] 

机构地区：[1]山东大学口腔医学院修复科·山东省口腔生物医学重点实验室,济南250012 [2] 第四军医大学口腔医学院修复科军事口腔医学国家重点实验室,西安710032 [3]济南军区总医院口腔科

出　　处：《中华口腔医学杂志》2014年第12期753-757,共5页Chinese Journal of Stomatology

基　　金：国家自然科学基金（81170985、81130078）

摘　　要：目的 比较分别去除牙本质蛋白聚糖和糖胺聚糖侧链后牙本质与全酸蚀粘接剂的即刻粘接性能,探索糖胺聚糖侧链或蛋白聚糖在牙本质粘接中的作用机制.方法 选择42颗无龋第三磨牙,制备标准牙本质粘接面,37％磷酸酸蚀15s后利用随机数字表法分为3组（每组14颗）,分别使用硫酸软骨素酶ABC （chondroitinase ABC,C-ABC）（C-ABC组,即去除糖胺聚糖）、胰蛋白酶（胰蛋白酶组,即去除蛋白聚糖）和去离子水（阴性对照组）37℃水浴振荡孵育48 h.处理后各组牙本质分别与全酸蚀粘接剂A（Adper TM Single Bond 2）和B（Primer＆Bond NT）制备标准粘接试件（每亚组6颗样本牙,制作20个粘接试件）,37℃水浴24 h后测试即刻粘接强度,分析断裂模式;观察粘接界面的微观形貌（每亚组1颗样本牙）.结果 C-ABC组牙本质与粘接剂A、B的即刻粘接强度[（32.9±2.5）和（26.8±2.2） MPa],与阴性对照组[（40.7±3.3）和（34.6±3.7） MPa]相比均显著下降（P＜0.05）;胰蛋白酶组牙本质与粘接剂A、B的即刻粘接强度[（49.0±3.6）和（44.5±3.0） MPa]均显著高于阴性对照组（P＜0.05）.结论 牙本质蛋白聚糖参与了牙本质的粘接过程,去除蛋白聚糖可提高粘接强度,去除糖胺聚糖侧链则降低牙本质与全酸蚀粘接剂的粘接强度.Objective To investigate the potential effect of proteoglycan（PG） and glycosaminoglycan（GAG） on the bonding of etch and rinse adhesive to dentin,in order to improve the bonding effect of dentin.Methods Forty-two extracted molars were used to obtain standard dentin bonding surface,and the specimens were etched for 15 s with 37% phosphoric acid and divided into three groups using a table of random numbers.Then the three groups undergone different incubating procedures as follow：specimens in chondroitinase ABC（C-ABC） group were incubated with C-ABC at 37 ℃ for 48 h in vibrator.Specimens in trypsin（TRY） group were incubated with trypsin,and specimens in the control group were incubated with deionized water for 48 h in the oscillators.Then specimens in each group were randomly assigned into two subgroups,A（AdperTM Single Bond 2） and B（Prime ＆ Bond NT） （n=7）.The microtensile bond strength（μTBS）,fracture mode and bonding interface morphology of the specimens were evaluated via microtensile testing,stereo microscope and field emission scanning electron microscopy（FE-SEM） respectively after specimens being incubated in 37 ℃ water for 24 h.Results The immediate μTBS of C-ABC group bonding with adhesive A and B [（32.9±2.5） and （26.8±2.2） MPa] were significantly lower than that of the control group [（40.7±3.3） and （34.6±3.7） MPa]（P〈0.05）.While the immediate μTBS of TRY group [（49.0± 3.6） and （44.5± 3.0） MPa] were significantly higher than that of the control group（P〈0.05）.Conclusions Dentin PG participates in the dentin bonding process.Removal of PG increased the immediate μTBS of dentin and total etching adhesives,while removal of GAG decreased the immediate μTBS.

关 键 词：牙本质 蛋白聚糖类 牙本质粘结剂 拉伸强度 

分 类 号：R783[医药卫生—口腔医学]