表没食子儿茶素没食子酸酯对小鼠精子畸形的影响  

Protective effect of epigallocatechin gallate against sperm abnormality in mice

作　　者：随刘才[1] 戈一峰[1] 许娟娟[1] 吴蓉花[1] 伏海燕[1] 姚兵[1] 

机构地区：[1]南京军区南京总医院生殖医学中心,江苏南京210002

出　　处：《中华男科学杂志》2014年第12期1068-1072,共5页National Journal of Andrology

摘　　要：目的:研究表没食子儿茶素没食子酸酯(EGCG)对实验小鼠雄性配子体内发育的影响。方法:选取64只6周龄昆明种雄鼠,随机分成如下8组:生理盐水组为阴性对照组,30 mg/kg环磷酰胺(CP)为阳性对照组,不同剂量EGCG组(20、40、80 mg/kg)以及EGCG抗环磷酰胺诱导组(30 mg/kg CP+20、40、80 mg/kg EGCG),隔日给药,连续5次。在首次给药的第4周和第5周,检测小鼠精子畸形率。结果:EGCG本身不会诱导小鼠精子畸形,而且延长EGCG的处理时间,小鼠精子畸形率有下降的趋势,尤其可以明显抑制CP诱导的小鼠精子畸形。结论:EGCG对在体内的雄性动物配子可能具有保护作用。Objective： To investigate the protective effect of epigallocatechin gallate （EGCG） on mouse sperm in vivo. Methods ： A total of 64 six-week-old male Kuming mice were randomly divided into eight groups of equal number to be treated with normal saline （ negative control）, Cyclophosphamide （CP） at 30 mg/kg （ positive control）, and CP followed by EGCG （experimental） at 20, 40, and 80 mg/kg, respectively, given every other day for 10 days. At 4 and 5 weeks after treatment, the bilateral testes of the mice were harvested for examination of sperm abnormality. Results ： EGCG did not increase the rate of CP-induced sperm abnormality in the mice, but reduced it instead with the prolonged time of treatment.

关 键 词：表没食子儿茶素没食子酸酯 环磷酰胺 精子畸形 小鼠 

分 类 号：Q492.4[生物学—生理学]