Crystal structure of the YTH domain of YTHDF2 reveals mechanism for recognition of N6-methyladenosine  

作　　者：Tingting Zhu Ian A Roundtree Ping Wang Xiao Wang Li Wang Chang Sun Yuan Tian Jie Li Chuan He Yanhui Xu 

机构地区：[1]Fudan University Shanghai Cancer Center, Department of Oncology and Institute of Biomedical Sciences and School of Basic Medical Sciences, Shanghai Medical College of Fudan University, Shanghai 200032, China [2]Medical Scientist Training Program and Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, 1L 60637, USA [3]Department of Chemistry and Institute for Biophysical Dynamics, The University of Chicago, Chicago, IL 60637, USA [4]Howard Hughes Medical Institute, The University of Chicago, Chicago, IL 60637, USA

出　　处：《Cell Research》2014年第12期1493-1496,共4页细胞研究（英文版）

基　　金：We thank staff members ofbeamline BL17U at SSRF （Shanghai Synchrotron Radiation Facility, China） for their assistance in data collection, and Mr Lei Zhang and staff members of Biomedical Core Facility in Fudan University for their help on biochemical analyses. We thank Dr Jinbiao Ma for the help on synthesis of m6A-RNA. This work was supported by grants from the National Basic Research Program of China （2011CB965300）, the National Science ＆ Technology Major Project ＂Key New Drug Creation and Manufacturing Program＂ of China （2014ZX09507-002）, the National Natural Science Foundation of China （U1432242, 91419301, 31270779 and 31030019）, the Basic Research Project of Shanghai Science and Technology Commission （12JC 1402700）, and the ＂Shu Guang＂ project （11SG06） supported by Shanghai Municipal Education Commission and Shanghai Education Development Foundation.

摘　　要：Dear Editor, N6-methyladenosine （m6A） has been demonstrated to be ubiquitous in several types of eukaryotic RNAs, including messenger RNA （mRNA）, transfer RNA （tRNA）, ribosomal RNA （rRNA）, long non-coding RNA （lncRNA）, and small nuclear RNA （snRNA） [1]. The recent discoveries of RNA m6A methyltransferase complex METTL3/METTL14/WTAP and demethylases FTO and ALKBH5 prove the reversibility of m6A modification [2-6]. This modification plays important roles in various biological processes, including circadian rhythms [7],

关 键 词：晶体结构 mRNA 识别 机制 核糖体RNA 非编码RNA 信使RNA 转移RNA 

分 类 号：Q78[生物学—分子生物学] O614.121[理学—无机化学]