An LC-MS/MS Method for the Simultaneous Determination of Lycorine and Galanthamine in Rat Plasma and Its Application to Pharmacokinetic Study of Lycoris Radiata Extract in Rats  

作　　者：周欣 刘月彬 黄珊 刘莹 

机构地区：[1]Department of Pharmacy, Fuzhou General Hospital of Nanjing Military Command

出　　处：《Journal of Huazhong University of Science and Technology(Medical Sciences)》2014年第6期861-868,共8页华中科技大学学报（医学英德文版）

基　　金：supported by the Natural Science Foundation of Fujian Province of China(No.2013J01382)

摘　　要：A rapid, sensitive, and selective liquid chromatography-tandem mass spectrometry was developed for the simultaneous determination of lycorine and galanthamine, two major constituents in Lycoris radiata extract, in rat plasma. Liquid-liquid extraction with ethyl ether was carried out using diphenhydramine as the internal standard The two bioactive alkaloids were separated on a Zorbax SB-C18 reserved-phase column （150 mm× 4.6 mm, i.d., 5 μm） by gradient elution using a mobile phase consisting of methanol with 0.1% formic acid （A） and water with 0.1% formic acid （B） at a flow rate of 0.6 mL/min. All analytes showed good linearity over a wide concentration range （r^2〉0.99） and the lower limit of quantification was 3.00 ng/mL for each analyte. The average extraction re- covery of the analytes from rat plasma was more than 82.15%, and the intra-day and inter-day accuracy and preci- sion of the assay were less than 12.6%. The validated method was successfully applied to monitoring the concen- trations and pharmacokinetic studies of two Amaryllidaceous alkaloids in rat plasma after an oral administration of Lycoris radiata extract.A rapid, sensitive, and selective liquid chromatography-tandem mass spectrometry was developed for the simultaneous determination of lycorine and galanthamine, two major constituents in Lycoris radiata extract, in rat plasma. Liquid-liquid extraction with ethyl ether was carried out using diphenhydramine as the internal standard The two bioactive alkaloids were separated on a Zorbax SB-C18 reserved-phase column （150 mm× 4.6 mm, i.d., 5 μm） by gradient elution using a mobile phase consisting of methanol with 0.1% formic acid （A） and water with 0.1% formic acid （B） at a flow rate of 0.6 mL/min. All analytes showed good linearity over a wide concentration range （r^2〉0.99） and the lower limit of quantification was 3.00 ng/mL for each analyte. The average extraction re- covery of the analytes from rat plasma was more than 82.15%, and the intra-day and inter-day accuracy and preci- sion of the assay were less than 12.6%. The validated method was successfully applied to monitoring the concen- trations and pharmacokinetic studies of two Amaryllidaceous alkaloids in rat plasma after an oral administration of Lycoris radiata extract.

关 键 词：liquid chromatography-tandem mass spectrometry Lycoris radiata extract PHARMACOKINETIC ratplasma 

分 类 号：R927.2[医药卫生—药学] O657.63[理学—分析化学]