A-NK细胞抗口腔舌鳞状细胞癌的实验研究  

Study of Anti-Tumor Effect of NK Cells on Oral Tongue Squamous Cell Carcinoma

作　　者：刘红[1] 祝军[1] 张真[1] 

机构地区：[1]新疆医科大学附属中医医院口腔科,830000

出　　处：《天津医药》2014年第12期1186-1189,共4页Tianjin Medical Journal

基　　金：自治区科技支疆项目(201191158)

摘　　要：目的观察黏附性自然杀伤(A-NK)细胞体外生长与增殖能力,探讨其治疗裸鼠舌鳞状细胞癌移植瘤的效果及其作用机制,为临床治疗鳞癌提供理论依据。方法抽取健康人外周血,分选出A-NK细胞和非黏附性自然杀伤(NA-NK)细胞体外培养,镜下观察细胞生长情况。建立裸鼠舌鳞癌皮下移植瘤模型,随机分成3组,每组7只小鼠,分别瘤旁注射生理盐水、A-NK细胞和NA-NK细胞,观察肿瘤体积变化,33 d后,处死裸鼠,剥离移植瘤,称瘤质量,绘制肿瘤生长曲线。结果镜下观察,A-NK细胞在第15天达到增殖高峰,NA-NK细胞于第12天达到增殖高峰。培养3周后,A-NK细胞共增加39.33倍,NA-NK细胞仅增加16.33倍。生理盐水组裸鼠口腔舌鳞癌移植瘤体积大于NA-NK细胞组和A-NK细胞组,NA-NK细胞组大于A-NK细胞组,3组裸鼠肿瘤体积在组间、时间和交互效应上差异均有统计学意义(P<0.01)。第33天,生理盐水组裸鼠瘤质量大于NA-NK细胞组和A-NK细胞组,NA-NK细胞组大于A-NK细胞组(P<0.05)。结论 A-NK细胞和NA-NK细胞均可在裸鼠体内诱导明显的抗肿瘤效应,且A-NK的抑瘤作用大于NA-NK细胞。Objective To explore the treatment effect and mechanism of A-NK cells on the subcutaneous trans-planted tumor of oral tongue squamous cell carcinoma in nude mice through observing the external growth and hyperplasia ofA-NK cells and to provide theoretical evidence for squamous cell carcinoma treatment. Methods A-NK cells and NA-NKcells were both derived from healthy human peripheral blood and cultured in vitro. Cell growth was observed under micro-scope. The squamous cell carcinoma model in nude mice was established through subcutaneous implanting of Tca8113 cells.Then they were randomly assigned into three groups who were injected with either saline solution, or A-NK cells or NA-NKcells paraneoplastically. All animals were sacrificed after 33 days when tumor were isolated then weight and change in tumorsize were assessed. Finally curve of tumor growth was drawn. Results Under the microscope, the proliferation of A-NKcells peak in 15 days and NA-NK cells peak in 12 days. After 3 weeks, the number of A-NK cells increased by 39.33 timeswhile the number of NA-NK cells increased by 16.33 times. The Volume of tongue squamous cell carcinoma in saline solu-tion group was larger than that in A-NK cells and NA-NK cells groups, and volume in the NA-NK cells group was largerthan that in A-NK cells group. The volume of tongue neoplasms in different groups, time, and interaction effects are statisti-cally significant（P〈0.01）. The tongue neoplasms weight in the saline solution group was greater than that in the A-NK cellsand NA-NK cells group, and the weight in NA-NK cells group was greater than that in A-NK cells group, and the differenceare statistically significant（P〈0.05）. Conclusion A-NK cells and NA-NK cells can significantly inhibit the subcutane-ous transplanted tumors in nude mice and the anti tumor effect of A-NK group is stronger than NA-NK.

关 键 词：杀伤细胞 天然 舌肿瘤 癌 鳞状细胞 A-NK细胞 NA-NK细胞 裸鼠移植瘤 抗肿瘤效应 

分 类 号：R739.8[医药卫生—肿瘤]