分散固相萃取-气相色谱-串联质谱法测定葡萄酒中56种农药残留  

Determination of 56 Pesticide Residues in Grape Wine by Gas Chromatography Tandem Mass Spectrometry Combined Modified QuEChERS Method

作　　者：吴凤琪[1] 沈金灿[1] 洪小柳[1] 吴卫东[1] 蓝芳[1] 

机构地区：[1]深圳出入境检验检疫局食品检验检疫技术中心/深圳市食品安全检测技术研发实验室,广东深圳518045

出　　处：《酿酒科技》2014年第12期88-93,共6页Liquor-Making Science & Technology

基　　金：国家质检总局科技计划项目资助(批准号:2010IK154和2012IK188);深圳市基础研究计划(项目编号:JC201105190972A)

摘　　要：建立葡萄酒中56种农药残留的气相色谱-串联质谱测定方法。样品经乙腈提取,经过PSA、C18和GCB等3种Qu ECh ERS吸附剂净化处理后,采用GC-MS/MS在选择反应监测离子（SRM）的模式下,进行质谱定性,内标法定量。结果表明,在5.0-100.0 g/L范围内,56种农药的回收率为69.0%-110.0%,相对标准偏差（RSD）均在15%以内,方法定量限为10 g/kg。所建立的方法简便快捷,试剂消耗量少,经济环保,可满足葡萄酒中农药残留量的实际检验需求。In this study, we established a method for determination of 56 pesticides residues in grape wine by gas chromatography tandem mass spectrometry. After sample preparation, the sample were extracted with acetonitrile. Clean-ap was carried out with three sorbents （PSA ,Ct8 and GCB）. The solution was determined and confirmed by GC-MS/MS, and quantitative analysis by internal standard method.The results showed that within the range of 5.0 - 100.0 g/L spiked level, the mean recoveries ranged from 69.0 % to 110.0 %, with the relative standard deviations （RSDs） under 15 %. The limits of quantifications （LOQs） was 10 g/kg. The proposed method is simple and efficient, and can meet the practical needs of grape wine inspection.

关 键 词：QUECHERS 葡萄酒 多农残 GC-MS/MS 

分 类 号：O657.63[理学—分析化学] TS262.6[理学—化学]