不同培养条件下空肠弯曲菌胞外多糖合成研究  

Study on Exopolysaccharide Syntheses of Campylobacter jejuni in Different States

作　　者：李安[1] 秦晓庆[1] 赵树臣[1] 侯振中[1] 

机构地区：[1]东北农业大学动物医学学院,黑龙江哈尔滨150030

出　　处：《动物医学进展》2014年第12期23-26,共4页Progress In Veterinary Medicine

基　　金：东北农业大学博士启动基金项目(2010RCB40);黑龙江省博士后科研启动金项目(LBH-Q11174)

摘　　要：为探讨空肠弯曲菌生物膜耐药机理,采用硫酸-蒽酮法测定了浮游菌和生物膜菌胞外多糖的产量。结果表明,两种状态细菌合成的水溶性胞外多糖量,随着时间的延长,呈现出先增加后下降的趋势。在同一时间点,生物膜菌黏附状态均显著低于浮游状态(P<0.05);水不溶性胞外多糖除了黏附状态合成量高于浮游状态外,不具有明显的规律。不同状态细菌合成胞外多糖差异显著,生物膜所处的酸性环境、营养物质的耗尽等可能是产生差异的原因。To investigate the mechanism of biofilm-resistant Campylobacter j ejuni ,sulfuric acid-anthrone method for the determination of planktonic bacteria and biofilm bacteria extracellular polysaccharide yields. The experiment showed that quantity of water soluble extracellular polysaccharide improved then decreased with the time lasted.At the same time,adhesion bacteria is significantly lower than planktonic bacteria,（P＆lt;0.05）,but the amouts of water insoluble extracellular polysaccharide were absolutely different.There was a significant differences of the ability in syntheses of exopolysaccharide between different conditions, reasons causing the differences may be acidic conditions where the biofilm grows and nutrients are deple-ted.

关 键 词：空肠弯曲菌 胞外多糖 硫酸-蒽酮法 

分 类 号：S852.613[农业科学—基础兽医学]