17β-雌二醇对双齿围沙蚕幼虫SOD、CAT和GST活性的影响  

Effect of 17β-estradiol on activities of SOD,CAT and GST in larvae of Perinereis aibuhitensis

作　　者：董学兴[1,2] 吕林兰[1] 吕富[1] 赵卫红[1] 於叶兵[1] 

机构地区：[1]盐城工学院海洋技术系,江苏省沿海池塘养殖生态重点实验室,江苏盐城224051 [2]上海海洋大学水产与生命学院,上海201306

出　　处：《南方水产科学》2014年第6期83-87,共5页South China Fisheries Science

基　　金：国家自然科学基金项目(31101887);江苏省自然科学基金项目(BK2012675;BK2011419)

摘　　要：将双齿围沙蚕(Perinereis aibuhitensis)幼虫暴露于不同质量浓度的17β-雌二醇(0.1μg·L-1、1μg·L-1、10μg·L-1、100μg·L-1和1 000μg·L-1)中,于第2、第4、第6和第8天取样,分别测定抗氧化酶[超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽硫转移酶(GST)]活性。结果表明,第2天时10μg·L-1浓度组显著诱导双齿围沙蚕CAT、SOD和GST活性,1 000μg·L-1浓度组显著抑制其SOD和GST活性。第4天时各浓度组SOD和GST活性均被不同程度诱导。第6天时低浓度组(0.1μg·L-1和1μg·L-1)显著抑制双齿围沙蚕CAT活性,高浓度组(100μg·L-1和1 000μg·L-1)则抑制其SOD活性。第8天时,除1 000μg·L-1浓度组外,其余各组CAT活性均被不同程度诱导(P>0.05),而SOD活性则受到抑制,10μg·L-1浓度组显著抑制其GST活性。试验结果显示,外源性17β-雌二醇对双齿围沙蚕幼虫产生氧化胁迫,沙蚕幼虫SOD、CAT、GST对17β-雌二醇的响应与其暴露浓度及时间有关。To evaluate the effect of 17β-estradiol on the antioxidative enzymes in larvae of Perinereis aibuhitensis,we exposed the worms to 17β-estradiol at different concentrations（ 0. 1 μg·L^-1,1 μg·L^-1,10 μg·L^-1,100 μg·L^-1and 1 000 μg·L^-1）,and measured the superoxide dismutase（ SOD）,catalase（ CAT） and glutathione S-transferase（ GST） activities on 2^nd,4^th,6^th and 8^th day,respectively. The results show that SOD,CAT and GST activities in 10 μg·L^-1treatment group increased significantly,and SOD and GST activities decreased notably in 1 000 μg·L^-1treatment group on 2^nd day. SOD and GST activities were induced at different degrees in every treatment group on 4^th day. CAT and SOD activities were suppressed in lower-concentration groups（ 0. 1 μg·L^-1and 1 μg·L^-1） on 6^th day and in higher-concentration groups（ 100 μg·L^-1and 1 000 μg·L^-1） on 6^th day,respectively（ P〈0. 05）. CAT activity was induced at different degrees in all groups except 1 000 μg·L^-1group（ P〉0. 05）; GST activity was suppressed in 10 μg·L^-1group; SOD activity was also suppressed in 17β-estradiol treatment groups on 8^th day. It is indicated that SOD, CAT and GST activities in P. aibuhitensis were induced by exogenous 17 β-estradiol,and the extent was related with the exposure concentration and time.

关 键 词：双齿围沙蚕 17Β-雌二醇 超氧化物歧化酶 过氧化氢酶 谷胱甘肽硫转移酶 

分 类 号：X174[环境科学与工程—环境科学]