Hippophae rhamnoides L.leaves extract enhances cell proliferation and neuroblast differentiation through upregulation of intrinsic factors in the dentate gyrus of the aged gerbil  

作　　者：Ji Hyeon Ahn Bai Hui Chen Joon Ha Park In Hye Kim Jeong-Hwi Cho Jae-Chul Lee Bing Chun Yan Jung Hoon Choi In Koo Hwang Ju-Hee Park Sang-No Han Yun Lyul Lee Myong Jo Kim Moo-Ho Won 

机构地区：[1]Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea [2]Department of Physiology, College of Medicine, Hallym University, Chuncheon 200-702, South Korea [3]Institute of Integrative Traditional & Western Medicine, Medical College, Yangzhou University, Yangzhou, Jiangsu 225001, China [4]Department of Anatomy, College of Veterinary Medicine, Kangwon NationaL University, Chuncheon 200-701, South Korea [5]Department of Anatomy and Cell Biology, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, South Korea [6]Samsung Herb Medicine Company Limitedl 304 BIO-ldong ChunCheon Biotechnology Innovation Center, Chuncheon 200-957, South Korea [7]Oriental Bio-herb Research Institute, Kangwon National University, Chuncheon 200-701, South Korea

出　　处：《Chinese Medical Journal》2014年第23期4006-4011,共6页中华医学杂志（英文版）

摘　　要：Background Hippophae rhamnoides L.（HL） exerts antioxidant activities against various oxidative stress conditions.In this study,we investigated effects of extract from HL leaves （HLE） on cell proliferation and neuroblast differentiation in the subgranular zone （SGZ） of the dentate gyrus （DG） of aged gerbils.Methods Aged gerbils （24 months） were divided into vehicle （saline）-treated-and HLE-treated-groups.The vehicle and HLE were orally administered with 200 mg/kg once a day for 20 days before sacrifice.Cell proliferation and neurobiast differentiation were examined in the DG using Ki67 and doublecortin （DCX）,respectively.We also observed changes in immunoreactivities of superoxide dismutase 1 （SOD1） and superoxide dismutase 2 （SOD2）,brain-derived neurotrophic factor （BDNF）,and phospho-glycogen synthase kinase-3-beta （p-GSK-3β） to examine their relation with neurogenesis using immunohistochemistry.Results The administration of HLE significantly increased the number of Ki67-positive cells and DCX-positive neuroblasts with well-developed processes in the SGZ of the DG of the HLE-treated-group.In addition,immunoreactivities of SOD1,SOD2,BDNF,and p-GSK-3β were significantly increased in granule and polymorphic cells of the DG in the HLE-treated-group compared with those in the vehicle-treated-group.Conclusions HLE treatment significantly increased cell proliferation and neuroblast differentiation,showing that immunoreactivities of SOD1,SOD2,BDNF,and p-GSK-3β were significantly increased in the DG.These indicate that increased neuroblast differentiation neurogenesis may be closely related to upregulation of SOD1,SOD2,BDNF,and p-GSK-3β in aged gerbils.Background Hippophae rhamnoides L.（HL） exerts antioxidant activities against various oxidative stress conditions.In this study,we investigated effects of extract from HL leaves （HLE） on cell proliferation and neuroblast differentiation in the subgranular zone （SGZ） of the dentate gyrus （DG） of aged gerbils.Methods Aged gerbils （24 months） were divided into vehicle （saline）-treated-and HLE-treated-groups.The vehicle and HLE were orally administered with 200 mg/kg once a day for 20 days before sacrifice.Cell proliferation and neurobiast differentiation were examined in the DG using Ki67 and doublecortin （DCX）,respectively.We also observed changes in immunoreactivities of superoxide dismutase 1 （SOD1） and superoxide dismutase 2 （SOD2）,brain-derived neurotrophic factor （BDNF）,and phospho-glycogen synthase kinase-3-beta （p-GSK-3β） to examine their relation with neurogenesis using immunohistochemistry.Results The administration of HLE significantly increased the number of Ki67-positive cells and DCX-positive neuroblasts with well-developed processes in the SGZ of the DG of the HLE-treated-group.In addition,immunoreactivities of SOD1,SOD2,BDNF,and p-GSK-3β were significantly increased in granule and polymorphic cells of the DG in the HLE-treated-group compared with those in the vehicle-treated-group.Conclusions HLE treatment significantly increased cell proliferation and neuroblast differentiation,showing that immunoreactivities of SOD1,SOD2,BDNF,and p-GSK-3β were significantly increased in the DG.These indicate that increased neuroblast differentiation neurogenesis may be closely related to upregulation of SOD1,SOD2,BDNF,and p-GSK-3β in aged gerbils.

关 键 词：aging ANTIOXIDANTS dentate gyrus hippophae rhamnoides extract NEUROGENESIS neurotrophic factors 

分 类 号：R285.5[医药卫生—中药学]