旋毛虫ES抗原对DC2.4细胞TLR4、NF-κB和AP-1 mRNA表达的影响  

Effect of ES antigen of Trichinella spiralis on expression of mRNA of TLR4,NF-κB and AP-1 in DC 2.4

作　　者：刘畅[1] 韩彩霞[1] 李巍[1] 李晓云[1] 路义鑫[1] 王泽[1] 唐颖[1] 宋铭忻[1] 

机构地区：[1]东北农业大学动物医学学院,哈尔滨150030

出　　处：《中国人兽共患病学报》2014年第11期1116-1120,共5页Chinese Journal of Zoonoses

基　　金：国家自然科学基金(No.31172312);"十一五"国家科技支撑计划重大项目(No.2010BAD04B01)联合资助~~

摘　　要：目的探讨旋毛虫ES抗原对小鼠DC2.4细胞TLR4、NF-κB和AP-1表达的影响。方法以实时荧光定量PCR(Real-time FQ-PCR)技术对空白对照组、LPS组、ES抗原组、ES抗原+LPS/ES抗原组和LPS+LPS/ES抗原组,检测TLR4、NF-κB和AP-1基因mRNA表达水平变化。结果 ES抗原和LPS单作用组的TLR4、NF-κB和AP-1mRNA相对表达量分别是6.14/4.09/3.63和4.68/4.71/4.11,是ES抗原和LPS预作用组的各基因相对表达量的2倍以上,差异有统计学意义(P<0.05)。结论实验证实,DC2.4细胞受ES抗原和LPS持续作用或者双重作用均可诱导小鼠DC2.4细胞TLR4、NF-κB和AP-1表达的降低,可能诱致DC2.4细胞免疫耐受。To explore the influence of Trichinella spiralis ES antigen on the expression of mRNA of TLR4,NF-κB and AP-1 in DC 2.4,we set up the following groups：blank control group,LPS group,ES antigen group,ES antigen＋LPS/ES antigen group,and LPS＋LPS/ES antigen group,which were presented by expression level of TLR4,NF-κB and AP-1 gene mRNA,and detected by fluorescence quantitative PCR （Real-time FQ-PCR） technology.Results showed that expression level of TLR4,NF-κB and AP-1 mRNA in pre-treatment of ES antigen and LPS group were significantly lower than that of ES and LPS group （P＜0.05）,respectively.We confirmed that the expression of mRNA of TLR4,NF-κB and AP-1 of DC2.4 could be suppressed by constant stimulation or combined effect of ES antigen and LPS.

关 键 词：旋毛虫ES抗原 TLR4 转录因子 实时荧光定量PCR 

分 类 号：R383.1[医药卫生—医学寄生虫学]