TCR γ δ/δ2在结直肠癌患者外周血和肿瘤组织中的表达及相关性  

Expression and Correlation of TCR γ δ/δ2 in Peripheral Blood and Tumor Tissue of Human Colorectal Cancer

作　　者：李文亮[1] 王志强[1] 文政琦[1] 殷亮[1] 杨军[1] 珠珠[1] 张洪斌[1] 

机构地区：[1]昆明医科大学第一附属医院肿瘤科,云南昆明650032

出　　处：《昆明医科大学学报》2014年第12期54-57,89,共5页Journal of Kunming Medical University

基　　金：云南省应用基础研究基金资助项目(2009CD085)

摘　　要：目的检测TCRαβ、TCRγδ及TCRδ2在结直肠癌患者外周血和肿瘤组织中的表达和相关性,为以γδT细胞为基础的免疫治疗提供依据.方法使用流式细胞仪检测TCRαβ、TCRγδ及TCRδ2三者在结直肠癌患者外周血、正常肠道粘膜组织、癌旁组织及癌组织中的表达;分析结直肠癌患者外周血中TCRδ2与组织中TCRγδ和TCRδ2表达水平的相关性.结果外周血中TCRγδ和TCRδ2在结直肠癌组中表达高于对照组(P<0.05).TCRγδ和TCRδ2在癌旁组织和癌组织中的表达高于正常肠道粘膜组织(P<0.05).当外周血TCRδ2升高到较高水平时,癌旁组织中TCRγδ和TCRδ2水平同步升高(P<0.05),而癌组织中TCRγδ和TCRδ2水平反而降低(P<0.05).结论结直肠癌患者外周血Vδ2 T细胞的水平与癌组织中浸润的γδT细胞和Vδ2 T细胞具有相关关系,为Vδ2 T细胞的局部介入治疗提供了理论依据.Objective To investigate the expression and correlation ofTCRαβ,TCRγδ and TCRδ2 in peripheral blood and tumor tissue of colorectal cancer patients,and provide evidence for immunotherapy based on γδT cell.Methods The expression of TCRαβ,TCRγδ and TCRδ2 in peripheral blood,normal intestinal mucosa,paracancerous and carcinoma tissue of colorectal cancer patient were detected with flow cytometry.The correlation between the expression of TCRδ2 in peripheral blood and TCRγδ,TCRδ2 in tissue was analyzed.Results The expression levels of TCR γδ,TCRδ2 in peripheral blood were obviously higher than that of the control group.The expression levels of TCRγδ,TCRδ2 in paracancerous and carcinoma tissue were higher than those of the nomal intestinal mucosa tissue.With the expression of TCRδ2 in peripheral blood at a high level the expression of TCR γδ and TCR δ2 in paracancerous tissues increased simultaneously,whereas the expression of TCR γδ and TCRδ2 declined in carcinoma tissue.Conclusions There are correlation between the level of Vδ2 T cell in peripheral blood and the level of γδ T cell and Vδ2 T cell in carcinoma tissue of colorectal cancer patient,which may provide theoretical evidence for regional intervention therapy of Vδ2T cell.

关 键 词：结直肠癌 ΓΔT细胞 外周血 肿瘤组织 过继免疫治疗 

分 类 号：R735.3[医药卫生—肿瘤]