脂多糖诱导成骨细胞MC3T3-E1中MMP-13表达的分子机制研究  

Study on molecular mechanism of expression of MMP-13 in lipopolysaccharide-induced osteoblast MC3T3-E1

作　　者：蔡淑娜 萧智利[2] 

机构地区：[1]舟山市妇幼保健院儿童口腔科,舟山316000 [2]重庆医科大学附属口腔医院牙周黏膜科,重庆400015

出　　处：《上海交通大学学报（医学版）》2014年第11期1599-1604,共6页Journal of Shanghai Jiao tong University:Medical Science

摘　　要：目的探讨脂多糖(LPS)对小鼠成骨细胞MC3T3-E1中基质金属蛋白酶-13(MMP-13)表达的影响及其分子机制。方法采用LPS处理传代培养的小鼠成骨细胞MC3T3-E1,Western blotting检测MC3T3-E1中MMP-13及转录因子C/EBPβ、核因子κB(NF-κB)及其抑制蛋白IκB、肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)的表达;观察使用NF-κB特异性抑制剂Bay11-7082干预及特异性siRNA沉默C/EBPβ、TNF-α和IL-6表达对MC3T3-E1中MMP-13表达的影响。结果 Western blotting检测结果显示:LPS能够诱导MC3T3-E1中MMP-13高表达,促进NF-κB、C/EBPβ、TNF-α和IL-6的表达和活化,抑制IκB表达;Bay11-7082干预及siRNA沉默TNF-α表达能有效抑制MC3T3-E1中TNF-α和MMP-13的表达,而siRNA沉默C/EBPβ和IL-6表达对MC3T3-E1中MMP-13的表达无明显影响。结论 LPS通过NF-κB/TNF-α诱导MMP-13在成骨细胞MC3T3-E1中高表达,这为阐明牙周炎的发病机制奠定了基础。Objective To investigate the effects of lipopolysaccharide （LPS） on the expression of matrix metaUoproteinases-13 （MMP-13） in mouse osteoblast MC3T3-E1 and their molecular mechanism. Methods The osteoblast MC3T3-E1 of subculture mouse was treated by LPS. Expressions of MMP-13 and transcription factor C/EBPβ, nuclear factor-KB （NF-KB） and its inhibitory protein Iv.B, tumor necrosis factor-α （TNF-α, and interleukin-6 （IL-6） in MC3T3-E1 cells were detected by the Western blotting. The effects of specific inhibitor Bay11-7082 of NF-KB and expressions of specific siRNA silencing C/EBPβ, TNF-α, and IL-6 on the expression of MMP-13 in MC3T3-E1 cells were observed. Results The results of Western blotting showed that LPS induced high level expression of MMP-13 in MC3T3-E1 cells, promoted the expression and activation ofNF-KB, C/EBP[3, and IL-6, and inhibited the expression of IKB. Expressions of TNF-α and MMP-13 in MC3T3-E1 cells could be effectively inhibited by Bayll-7082 and sLRNA silencing TNF-α. While the siRNA silencing C/EBPβ and the expression of IL-6 did not significandy influence the expression of MMP-13 in MC3T3-E1 cells. Conclusion LPS can induce high level expression of MMP-13 in osteoblast MC3T3-E1 by NF-KB/TNF-α, which helps clarifying the pathogenesis of periodontitis.

关 键 词：脂多糖 基质金属蛋白酶-13 肿瘤坏死因子-α 牙周炎 

分 类 号：R781.4[医药卫生—口腔医学]