高效液相色谱法测定藤茶中二氢杨梅素和杨梅素含量  

Content Determination of Dihydromyricetin and Myricetin in Leaves of Ampelopsis Grossedentata by HPLC

作　　者：陈图锋[1,2] 高文华[1,2] 唐敏[2] 

机构地区：[1]汕头大学分析测试中心,广东汕头515063 [2]汕头大学化学系,广东汕头515063

出　　处：《亚太传统医药》2015年第1期28-30,共3页Asia-Pacific Traditional Medicine

基　　金：广东省自然科学基金资助项目(S2011010005208);广东省教育厅科技创新项目(2013KJCX0078)

摘　　要：目的：建立以高效液相色谱法同时测定藤茶中二氢杨梅素和杨梅素含量的方法。方法：采用高效液相色谱法。色谱柱为SUPELCO Discovery C18 HPLC Column（25cm×4．6mm，5μm）；流动相为甲醇-0．03％磷酸溶液，采用梯度洗脱，流速为0．8mL·min，杨梅素和二氢杨梅素检测波长分别为373nm和290nm。结果：藤茶中二氢杨梅素和杨梅素分别在0．15～94．0mg·L^-1（R2=1）和0．62～77．50mg·L^-1（R^2=0．9997）浓度范围内有良好线性关系；平均加标回收率分别为89．7％、93．5％，RSD〈4．5％。结论：该法简便、快速、准确，可用于藤茶的质量标准研究及其质量控制。Objective： To develop a method for the content determination of dihydromyricetin（DMY） and myricetin（ MY） in leaves of Arnpelopsis grossedentata. Methods： HPLC method was adopted. The separation was performed on SUPELCO Discovery C18 （25cm × 4.6mm, 5μm）column with 0.03% phorsphoric acid-methanol as mobile phase at flow rate of 0.8 mL· min-1. The detection wavelength of MY was set at 373 nm,and that of DMY was set at 290 nm. Results：The linear range was 0.15～94mg ·L^-1 （R2= 1）for DMY and 0.62～77.50mg · L-1 （R2=0. 999 7） for MY with average recovery of 89.7% and 93.5% ,RSD〈4.5%. Conclusion：The method is simple,fast,accurate aprovide the basis for the quality standard of leaves of A. grossedentata.

关 键 词：藤茶 二氢杨梅素 杨梅素 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学]