Effects of Acupuncture on Progesterone and Prolactin in Rats of Embryo Implantation Dysfunction  

作　　者：熊繁 桂娟 杨薇 李婧 黄光英 

机构地区：[1]Institute of Integrative Traditional Chinese and Western Medicine,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology [2]Institute of Obstetrics and Gynecology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology [3]Institute of Reproductive Medicine,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology

出　　处：《Chinese Journal of Integrative Medicine》2015年第1期58-66,共9页中国结合医学杂志（英文版）

基　　金：Supported by National Nature Science Foundation of China(No.S-049-09-10-20);National Basic Research Program of China(973 Program,No.2011CB505203)

摘　　要：Objective： To investigate the effect of acupuncture on progesterone （P4） and prolactin （PRL） in rats of embryo implantation dysfunction （EID）. Methods： On the first day of pregnancy, 72 female Wistar rats were randomly allocated into the normal group, the EID model group, the acupuncture group and the P4 group （18 in each group）. The normal group was injected sesame oil, while the other three groups were given mifepristone to establish the EID model. The acupuncture group and the P4 group were given treatment of acupuncture and P4 injection, respectively. The serum of P4 and PRL were detected by radioimmunoassay, and the mRNA and protein expressions of P4 receptor （PR） and PRL receptor （PRLR） were detected using real-time polymerase chain reaction and immunohistochemical method, respectively. Results： Compared with the normal group, the serum levels of P4 and PRL as well as the mRNA and protein expression levels of PR and PRLR in the EID model group were significantly lowered （P〈0.01 or P〈0.05）. The above indices in the acupuncture group and the P4 group were significantly elevated compared with the EID model group （P〈0.01 or P〈0.05）. Conclusion： Acupuncture can promote embryo implantation effectively, which might be related to the effects of acupuncture on upregulating the P4 and PRL levels in serum and the PR and PRLR expression levels in rats.Objective： To investigate the effect of acupuncture on progesterone （P4） and prolactin （PRL） in rats of embryo implantation dysfunction （EID）. Methods： On the first day of pregnancy, 72 female Wistar rats were randomly allocated into the normal group, the EID model group, the acupuncture group and the P4 group （18 in each group）. The normal group was injected sesame oil, while the other three groups were given mifepristone to establish the EID model. The acupuncture group and the P4 group were given treatment of acupuncture and P4 injection, respectively. The serum of P4 and PRL were detected by radioimmunoassay, and the mRNA and protein expressions of P4 receptor （PR） and PRL receptor （PRLR） were detected using real-time polymerase chain reaction and immunohistochemical method, respectively. Results： Compared with the normal group, the serum levels of P4 and PRL as well as the mRNA and protein expression levels of PR and PRLR in the EID model group were significantly lowered （P〈0.01 or P〈0.05）. The above indices in the acupuncture group and the P4 group were significantly elevated compared with the EID model group （P〈0.01 or P〈0.05）. Conclusion： Acupuncture can promote embryo implantation effectively, which might be related to the effects of acupuncture on upregulating the P4 and PRL levels in serum and the PR and PRLR expression levels in rats.

关 键 词：ACUPUNCTURE PROGESTERONE PROLACTIN progesterone receptor prolactin receptor embryo implantation dysfunction 

分 类 号：R245[医药卫生—针灸推拿学]