百合多糖联合金雀异黄素对人类乳腺癌细胞增殖的影响  

Effect of lily polysaccharide combined genistein on proliferation and cell cycle in human breast carcinoma cells

作　　者：侯进[1] 李汾[1] 李新华[1] 梅其炳[2] 弥曼[1] 

机构地区：[1]西安医学院药理学与毒理学教研室,陕西西安710021 [2]第四军医大学药理学教研室,陕西西安710032

出　　处：《现代肿瘤医学》2015年第1期12-14,共3页Journal of Modern Oncology

基　　金：陕西省教育厅专项科研项目(编号:2010JK805);西安医学院博士科研启动基金项目(编号:2011DOC04);西安医学院扶持基金项目(编号:2009FZ2)

摘　　要：目的:研究百合多糖(LP-1)联合金雀异黄素对人类乳腺癌细胞增殖的影响。方法:培养MCF-7细胞,加入不同浓度的LP-1和金雀异黄素,应用MTT法检测百合多糖对MCF-7细胞增殖的影响;用流式细胞技术(FCM)检测细胞周期。放射性配体结合法检测化合物与雌激素受体(estrogenic receptor,ER)的亲和力。结果:LP-1对MCF-7细胞增殖和细胞周期没有显著影响,和高浓度金雀异黄素合用可以增强金雀异黄素对MCF-7细胞的抗增殖作用,并将细胞周期阻滞在G2期。LP-1对ERα和ERβ均无亲和力,金雀异黄素对ERα和ERβ均有亲和力,IC50分别(3.51×10-5)mol/L和(2.12×10-7)mol/L。结论:百合多糖与金雀异黄素合用可能通过不同途径影响MCF-7细胞增殖和凋亡,发挥协同作用。Objective：To investigate the effects of lily polysaccharide（LP-1）combined genistein on the prolifera-tion of mammary cancer cells in vitro. Methods：Effects of LP-1 and genistein on the cell proliferation were tested in MCF-7 cell by MTT measurement. Cell cycle was examined by flow cytometry. Competitive estrogen-receptor bind-ing assay was used to investigate the affinity of xy2004 to ER. Results：LP-1 used alone can′t inhibit proliferation of MCF-7 cell,while proliferation of MCF-7 cell was inhibited when LP-1 and genistein used together. The cell cy-cle was arrested to G2 by using polysaccharide and genistein together. The relative binding affinity of genistein for ERαand ERβ showed an IC50 of（3. 51 × 10-5 ）mol/L and（2. 12 × 10-7 ）mol/L respectively. Conclusion：LP-1 and genistein have the synergistic antitumor action through the different response pathway.

关 键 词：MCF-7细胞 百合多糖 金雀异黄素 

分 类 号：R73-361[医药卫生—肿瘤] R737.9[医药卫生—临床医学]