顺铂间歇性干预对A549中干细胞及EMT相关基因表达的影响  

Effect of intermittent cisplatin on expression of stem cells and EMT- related genes in A549 cell lines

作　　者：王红艳[1] 张雅洁[1] 

机构地区：[1]广州医科大学病理教研室,广东广州510182

出　　处：《现代肿瘤医学》2015年第2期162-165,共4页Journal of Modern Oncology

基　　金：教育部博士点基金博导类课题(编号:20134423110001);广东省自然科学基金(编号:S2012010010181);广州市科技计划项目(编号:2014Y2-001 71);广州市教育系统创新学术团队项目(编号:13C06);广州市属高校科研项目(编号:2012C135)

摘　　要：目的:研究肺癌细胞A549在顺铂(DDP)间歇性干预后肿瘤干细胞(CSC)标志物(CD133)和上皮细胞间质转化(EMT)标志物(E-cadherin、β-catenin、Vimentin和Fibronectin)表达的变化,探讨肺癌耐药和CSC及EMT之间的相关性。方法:应用CCK-8法检测A549细胞对DDP的敏感性,DDP IC50间歇性处理A549细胞,采用Western blot法检测CD133蛋白表达变化,RT-q PCR法测定A549细胞处理前后E-cadherin、β-catenin、Vimentin和Fibronectin mRNA的表达。结果:肺腺癌细胞A549经DDP间歇性干预后,CSC标记物CD133蛋白表达增高,E-cadherin和β-catenin mRNA的相对表达量均显著降低,Vimentin mRNA的相对表达量显著增高。结论:A549细胞经DDP间歇性干预后有CSC标志物的表达,并表现出EMT的特性。Objective：To observe the impacts of intermittent cisplatin （DDP） on expression of cancer stem cell marker（CD133） and EMT markers （E-cadherin,β-catenin,Vimentin and Fibronectin） in lung cancer A549 cell lines,and explore the correlations of drug resistance,cancer sternness and EMT.Methods：The drugs sensitivity of A549 cells to cisplatin was detected by CCK-8.A549 cells were treated with cisplatin IC5o using interval-inactivation methods,CD133 protein expression of A549 cells in pre-and post-chemotherapy was detected by Western blot,and the expression of E-cadherin,β-catenin,Vimentin and Fibronectin was detected by Real-time PCR.Results：After treatment with intermittent cisplatin,the expression of CD133 in A549 cells was significantly enhanced,the relative expression of E-cadherin,β-catenin mRNA was significantly decreased,while the relative expression of Vimentin mRNA was significantly increased.Conclusion：After interval treatment with cisplatin,there is expression of cancer stem cell markers in A549 cells,which exhibit characteristics of EMT.

关 键 词：肺癌 耐药 肿瘤干细胞 上皮细胞间质转化 

分 类 号：R734.2[医药卫生—肿瘤]