5-氮-2'-脱氧胞苷对肺癌A549细胞凋亡及14-3-3σ表达的影响  

Effect of 5- Aza- CdR on cell apotosis in lung cancer A549 cell line and expression of tumor suppressor gene 14- 3- 3σ

作　　者：余宗涛[1] 张吉才[1] 高琼[2] 高波[1] 胡春卉[1] 

机构地区：[1]湖北医药学院附属十堰市太和医院检验科,湖北十堰442000 [2]湖北医药学院附属十堰市太和医院呼吸内科,湖北十堰442000

出　　处：《现代肿瘤医学》2015年第2期170-173,共4页Journal of Modern Oncology

基　　金：湖北省十堰市科技局项目(编号:ZD2012015);湖北医药学院优秀中青年科技创新项目(编号:2011 CXG02)

摘　　要：目的:探讨5-氮-2'脱氧胞苷(5-Aza-CdR)对人肺癌A549细胞凋亡及抑癌基因14-3-3σ表达的影响。方法:以浓度为0.5、5、50μmol/L的5-Aza-CdR处理人肺癌细胞株A549,常规培养采用四唑盐(MTT)比色法观察细胞的生长活性,甲基化特异性聚合酶链反应(MSP)检测14-3-3σ基因甲基化状态;以FQ-PCR法检测14-3-3σmRNA的表达,并用流式细胞仪检测细胞凋亡率。结果:5-Aza-CdR能明显抑制肿瘤细胞的生长,随5-Aza-CdR浓度增加及培养时间的延长,细胞生长率下降;药物处理后14-3-3σmRNA表达明显升高,细胞凋亡率与5-Aza-CdR剂量呈正相关。结论:5-Aza-CdR能使14-3-3σ基因去甲基化、促进细胞凋亡、增强抑癌功能。Objective：To investigate the effect of 5-Aza-2＇-deoxycytidine （5-Aza-CdR） on the apoptosis of A549 cells and the expression of 14-3-3σ gene.Methods：A549 cells were treated with 5-Aza-CdR（0.5,5,50μmol/L respectively）.The growth of A549 cells was observed by MTT assay respectively.The methylation status of 14-3-3σ gene was observed by methylation specific PCR （MSP） ;the expression of 14-3-3σ mRNA was observed by FQ-PCR.The apoptosis rate of A549 cells was analyed by flow cytometry.Results：A549 cells treated with 5-Aza-CdR displayed a slow growth in comparison with the control cells,and the growth rate decreased with the increasing of 5-Aza-CdR concentration.14-3-3σ mRNA expression increased in a 5-Aza-CdR concentration dependent（P ＜ 0.05）.The apoptosis rates after treatment were higher than those before treatment in A549 cells（P ＜0.05）,and had a positive correlation with 5-Aza-CdR dose.Conclusion：5-Aza-2＇-CdR can induce the apoptosis of A549 cells by inducing demethylation and thereby enchancing 14-3-3σ gene,enhancing tumor suppressor function.

关 键 词：14-3-3Σ基因 甲基化 5-氮-2脱氧胞苷 A549细胞 凋亡 

分 类 号：R734.2[医药卫生—肿瘤]