顺铂诱导鼻咽癌CNE-2细胞自噬和凋亡的体外研究  

In vitro study on the relationship between autophagy and apoptosis induced by cisplatin in human pharyngeal carcinoma cell line CNE- 2

作　　者：陈向军[1] 刘国磊[1] 郜元坤[1] 田磊[1] 李国义[1] 

机构地区：[1]十堰市太和医院耳鼻喉头颈外科,湖北十堰442000

出　　处：《现代肿瘤医学》2015年第2期180-185,共6页Journal of Modern Oncology

摘　　要：目的:研究顺铂在体外应用对人鼻咽癌CNE-2细胞凋亡与自噬的影响,并探讨其可能机制。方法:采用流式细胞术检测细胞周期分布、细胞凋亡率和MDC阳性率;RT-PCR法检测Mcl-1、Bcl-2、Bad、Bax、Caspase-3、Beclin1和LC3 mRNA表达。结果:在实验浓度范围内,顺铂作用CNE-2细胞48h后,2、4、8μg/ml各干预组总凋亡率分别为(8.71±1.03)%、(11.34±1.17)%、(15.30±2.24)%;MDC阳性率分别为(5.36±0.48)%、(7.52±0.32)%、(10.65±0.70)%;与0μg/ml组比较,G2/M期阻滞率分别增加45.97%、73.99%、267.34%;Bcl-2和Mcl-1 mRNA表达剂量依赖性降低,Bax、Bad、Caspase-3、Beclin1和LC3 mRNA表达剂量依赖性增加,且4μg/ml和8μg/ml与0μg/ml组比较,以上研究指标差别均有统计学意义(P<0.05)。结论:顺铂可诱导鼻咽癌CNE-2细胞发生G2/M期阻滞、细胞凋亡和自噬性细胞死亡,对CNE-2细胞具有杀伤作用,其分子机制可能与Bcl-2表达下调和Caspase-3、Beclin1表达上调有关。Objective：To explore the relationship between autophagy and apoptosis induced by cisplatin（DDP） in human pharyngeal carcinoma cell line CNE-2 in vitro,the possible mechanism of this technique is also evaluated in this study.Methods：The apotosis rates,changes of cell cycle and positive rates of MDC were determined by flow cytometry.RT-PCR was applied to detect the expression of Mcl-1,Bcl-2,Bad,Bax,Caspase-3,Beclin1 and LC3.Results：After CNE-2 cells were treated with different concentrations of DDP for 48 hours,the apoptosis rate of 2,4,8μ g/ml intervention groups were（8.71 ±-1.03） ％,（11.34 ± 1.17） ％ and （15.30 ± 2.24） ％ ; MDC positive rate were （5.36 ± 0.48） ％,（7.52 ± 0.32） ％ and （10.65 ± 0.70） ％ ; compare with 0μg/ml group,G2/M phase cell cycle arrest rate increased by 45.97％,73.99％ and 267.34％ respectively.The expression of Bcl-2 and Mcl-1 decreased in aconcentration-dependent manner,meanwhile,the expression of Bax,Bad,Caspase-3,Beclin1 and LC3 increased in adose-dependent manner; 4μg/ml group and 8μ g/ml group compared with 0μg/ml group,there were significantly statistical differences of all above index.Conclusion：DDP could induce apoptosis,G2/M cell cycle arrest and autophagy in the CNE-2 cells,DDP could inhibit proliferation of CNE-2 cells.The involved mechanisms may be associated with decreased expression of Bc1-2 and enhanced expression of Caspase-3 and Beclin1.

关 键 词：鼻咽肿瘤 鼻咽癌CNE-2细胞 顺铂 细胞凋亡 细胞周期 自噬 

分 类 号：R739.6[医药卫生—肿瘤]