TGF-β1、CD34和ɑ-SMA在鼻咽癌中的表达及其意义  

Expression and significance of TGF- β1,CD34 and α- SMAin nasopharyngeal carcinoma

作　　者：莫立根[1] 罗元[2] 党阳[1] 邓腾[1] 张堃[1] 王琳[1] 

机构地区：[1]广西医科大学附属肿瘤医院头颈外科,广西南宁530021 [2]广西医科大学附属肿瘤医院病理科,广西南宁530021

出　　处：《现代肿瘤医学》2015年第2期185-188,共4页Journal of Modern Oncology

基　　金：广西自然科学基金资助项目(编号:2011GXNSFA018247);广西卫生厅重点课题资助项目(编号:重2010069)

摘　　要：目的:探讨转化生长因子β1(TGF-β1)、肿瘤相关纤维母细胞相关蛋白CD34和平滑肌肌动蛋白(α-SMA)表达与鼻咽癌侵袭转移的关系。方法:采用免疫组化Supervision二步法检测75例鼻咽癌患者和20例鼻咽黏膜慢性炎症患者鼻咽组织中TGF-β1、CD34和α-SMA表达。结果:TGF-β1、α-SMA在鼻咽癌组织中表达阳性率均明显高于鼻咽黏膜慢性炎组织中表达阳性率(P<0.05),而CD34在鼻咽黏膜慢性炎组织中的表达阳性率显著高于在鼻咽癌组织中表达阳性率(P<0.05)。TGF-β1、α-SMA表达与性别无关(P>0.05),与鼻咽癌TNM分期、淋巴结转移和远处转移有关(P<0.05);鼻咽癌组织中,TGF-β1与α-SMA表达呈正相关(r=0.368,P<0.01),与CD34表达呈负相关(r=-0.397,P<0.01);α-SMA与CD34表达呈负相关(r=-0.434,P<0.01)。结论:肿瘤相关纤维母细胞相关蛋白可能与鼻咽癌侵袭转移密切相关。与鼻咽黏膜慢性炎组织中的成纤维母细胞相比,鼻咽癌细胞可能通过分泌TGF-β1作用于肿瘤间质,促使肿瘤相关纤维母细胞CD34表达缺失,而高表达α-SMA,通过表型改变为肌纤维母细胞。Objective：To study the expression of TGF-β1,α-SMA and CD34 by immunohistochemistry in patients with nasopharyngeal carcinoma （NPC） and nasopharyngeal inflammation tissues.Methods：The expression of TGF-β1,α-SMA and CD34 proteins in nasopharyngeal carcinoma（n =75） and nasopharyngeal inflammation tissues（n =20） was examined by immunohistochemistry.Results：The positive rates of TGF-β1 and α-SMA protein in NPC tissues were significantly higher than those in nasopharyngeal inflammation tissues （P ＜ 0.05）.The positive rate of CD34 protein in nasopharyngeal inflammation tissues was significantly higher than those in NPC tissues（P ＜ 0.05）.The overexpression of TGF-β1 and α-SMA protein in NPC was significantly correlated to clinical stage,lymph node metastasis and distant metastasis （P ＜ 0.05）,but had no relationship with gender（P ＞ 0.05）.TGF-β1 was positively associated with α-SMA （r =0.368,P ＜ 0.01）,but TGF-β1 was negatively associated with CD34 （r =-0.397,P ＜ 0.01）,and α-SMA was negatively associated with CD34 （r =-0.434,P ＜ 0.01）.Conclusion：The overexpression of carcinoma-associated fibroblasts protein is related to invasion and metastasis of NPC.Fibroblasts in the nasopharyngeal carcinoma interstitium show phenotypic differences from the fibroblasts of chronically inflamed nasopharyngeal mucosa.The transdifferentiation of fibroblasts to CAFs is driven to a great extent by cancer-derived TGF-β1.These changes include lack of CD34 expression and overexpression of α-SMA.

关 键 词：鼻咽癌 肿瘤相关纤维母细胞 转化生长因子β1 白细胞分化抗原34 Α-平滑肌肌动蛋白 

分 类 号：R739.6[医药卫生—肿瘤]