平阳霉素联合透明质酸钠对人淋巴管畸形内皮细胞的作用  

Effects of pingyangmycin combined with sodium hyaluronate on human lymphatic malformation endothelial cells

作　　者：赵仪云[1] 黄敬春[1] 王月莲[1] 邵山[1] 孙广平[1] 崔恩美[1] 卓绍杨[2] 刘少华[3] 

机构地区：[1]济南市第三人民医院口腔科,山东济南250101 [2]山东大学口腔医院口腔颌面外科,山东济南250012 [3]山东大学齐鲁医院口腔科,山东济南250012

出　　处：《山东大学学报（医学版）》2015年第1期41-46,共6页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金(81172572);山东省自然科学基金(ZR2010HM008);济南市科技发展计划[2011]34号

摘　　要：目的探讨平阳霉素(PYM)联合透明质酸钠(HA)对体外培养的人淋巴管畸形内皮细胞(HLMECs)的作用。方法对新鲜婴幼儿淋巴管畸形组织进行HLMECs原代培养。利用淋巴管内皮特异性标志物LYVE1和PROX1染色,将不同浓度(1、10、100、500μg/m L)的PYM和(50、100、300、500μg/m L)HA分别作用于HLMECs 12、24、48 h,观察对HLMECs增殖的影响并筛选其最适有效浓度。然后用该有效浓度的PYM和HA共同作用于HLMECs,在第24小时时,MTT法检测细胞生长情况,TUNEL法检测细胞凋亡情况。结果 1淋巴管畸形组织和HLMECs表达LYVE1和PROX1;2PYM的最适浓度为10μg/m L,HA的最适浓度为100μg/m L;3PYM联合HA在抑制细胞增殖和促进细胞凋亡方面比单纯的PYM作用强。结论 PYM联合HA有望在临床上用于治疗淋巴管畸形。Objective To explore the effects of pingyangmycin （PYM）combined with sodium hyaluronate （HA）on human lymphatic malformations endothelial cells （HLMECs）in vitro.Methods Primary HLMECs were isolated from a HLMs tissue and cultured with endothelial cell medium （ECM）.By lymphatic endothelial specific marker LYVE1 and PROX1 staining,the HLMs specimen and HLMECs were identified.The proliferation effects of HLMECs treated with gradient densities of PYM （1,10,100,500 μg/mL）or HA （50,100,300,500 μg/mL）for 12,24 and 48 hours,were compared with MTT assay,and the optimal concentration of PYM and HA were selected.After that,the HLMECs were treated with PYM and HA of the optimal concentration.After 24 hours,the cell proliferation and apop-tosis were evaluated with MTT assay and TUNEL respectively.Results ① Both LYVE1 and PROX1 were expressed in the HLMs tissue and HLMECs.② The optimal concentration of PYM and HA was 10 μg/mL and 100 μg/mL respectively.③ The combination of PYM and HA was more effective than PYM alone on inhibiting HLMECs growth and promoting HLMECs apoptosis.Conclusion The combination of PYM and HA is effective for HLMECs,which can be an alternative in the clinical treatment of lymphatic malformations.

关 键 词：平阳霉素 透明质酸钠 淋巴管畸形内皮细胞 

分 类 号：R782[医药卫生—口腔医学]