HPLC指纹法评价白芷饮片质量的一致性  

Evaluation of Quality Coherence of Angelicae Dahuricae Radix(Baizhi) Decoction Pieces Based on HPLC Fingerprint

作　　者：吴晓毅[1] 巢志茂[1] 王淳[1,2] 于莉[1,3] 梁曜华[1] 孙文[1] 廖永红[3] 

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]中国中医科学院中医基础理论研究所,北京100700 [3]北京工商大学食品学院,北京100048

出　　处：《中国中医药信息杂志》2015年第2期74-78,共5页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：人力资源和社会保障部留学人员科技活动项目(人社厅函〔2011〕508号);中医药行业科研专项(201407005);中国中医科学院博士研究生创新人才培养基金(2014年)

摘　　要：目的 对全国市售白芷饮片进行质量-致性的评价.方法 采用高效液相色谱法（HPLC）测定12 份不同地区的市售饮片、1 份硫磺熏蒸药材和1 份自然晾干药材的指纹图谱;测定饮片中欧前胡素和异欧前胡素的含量.HPLC 条件为：C18色谱柱（4.6 mm×250 mm,5 μm）;流动相为乙腈-水,梯度洗脱,流速1.0 mL/min;柱温35 ℃;检测波长选择210～800 nm 范围的最大绘图.通过相似度计算方法进行分析比较.结果 建立了白芷饮片的HPLC 指纹图谱分析方法,确定了12 个共有特征峰.市售饮片的相似度为0.840～0.973;与硫磺熏蒸药材相比,指纹图谱的相似度为0.672～0.908;与自然晾干药材相比,指纹图谱的相似度为0.536～0.684.市售饮片中欧前胡素和异欧前胡素的含量范围分别为0.035%～0.140%和0.028%～0.069%.结论 不同地区的市售白芷饮片具有质量一致性,推测饮片均为硫磺熏蒸品.Objective To evaluate the quality coherence of commercial Angelicae Dahuricae Radix (Baizhi) decoction pieces. Methods The samples of 12 batches of commercial decoction pieces, 1 batch of sulphur fumigated Baizhi, and 1 batch of naturally dried Baizhi were collected. HPLC method was used to determine the fingerprints and the contents of imperatorin and isoimperatorin by C18 column (4.6 mm×250 mm, 5 μm) with a gradient mobile phase of acetonitrile-water solution system at the flow rate of 1.0 mL/min. The column temperature was set at 35 ℃, and the max plot of detection wavelength was in 210-800 nm. Similarity calculation was used to analyze the data. Results The HPLC fingerprint analysis method was established with 12 common peaks. The similarities of 12 batches of commercial decoction pieces of Baizhi were 0.840-0.973. Their similarities compared with the sulphur fumigated Baizhi were 0.672-0.908. Compared with naturally dried Baizhi, the similarities of fingerprint were 0.536-0.684. The contents of imperatorin and isoimperatorin in 12 batches of commercial decoction pieces were 0.035%-0.140% and 0.028%-0.069%, respectively. Conclusion The quality of Baizhi decoction pieces was consistent. It can be speculated that Baizhi decoction pieces were processed with sulphur fumigation.

关 键 词：白芷 指纹图谱 饮片 欧前胡素 异欧前胡素 质量一致性 

分 类 号：R284.1[医药卫生—中药学]