草鱼呼肠孤病毒096 vp7基因生物信息学分析及其酵母表达载体的构建  

Construction of Yeast Expression Vector and Bioinformatics Analysis of vp7 Gene in GCRV 096

作　　者：郑树城[1] 谢吉国[1,2] 王雅[1] 蔡双虎[1] 简纪常[1] 吴灶和[1] 闫秀英[1] 

机构地区：[1]广东海洋大学水产学院//广东省水产经济动物病原生物学及流行病学重点实验室暨广东省高等学校水产经济动物病害控制重点实验室,广东湛江524088 [2]北京市大兴区水产技术推广站,北京102600

出　　处：《广东海洋大学学报》2014年第6期31-37,共7页Journal of Guangdong Ocean University

基　　金：国家973计划项目(2009CB118704)

摘　　要：草鱼呼肠孤病毒(Grass carp reovirus,GCRV)是草鱼出血病的病原。从患病草鱼体内分离到一株草鱼呼肠孤病毒GCRV 096,经反转录PCR,克隆得GCRV 096长度为855 bp的vp7基因,并分析该基因编码蛋白的特性。结果表明,同一基因型分离株VP7蛋白间的差异不大,但不同基因型分离株VP7蛋白间存在很大的差异。对GCRV 096 VP7蛋白生物信息学分析表明,信号肽位点位于20氨基酸处,且VP7含有4个潜在的抗原决定簇。构建GCRV 096 vp7基因的酵母表达载体p GBKT7-S10,并成功转化至酵母中。Grass Carp Reovirus （GCRV） is the aetiological agent of grass carp hemorrhage. GCRV096 was isolated from the diseased grass carp. After RT-PCR, the vp7 gene in GCRV 096, 855 bp inlength, was cloned. Characteristics of the VP7 protein, encoded by the vp7 gene, were analyzed.The results showed that there was very little difference of the VP7 proteins in GCRV strains of thesame genotype, but there was much difference in that of GCRV strains of different genotypes.Bioinformatics analysis of the VP7 protein indicated that the signal peptide site was located at 20amino acid, and the VP7 protein had four potential antigenic determinants. Furthermore, yeastexpression vector pGBKT7-S10 of the vp7 gene was constructed, and successfully transformed intoyeast cells.

关 键 词：草鱼呼肠孤病毒 VP7基因 生物信息学 酵母表达载体 

分 类 号：Q78[生物学—分子生物学] Q939.4