埃博霉素B高产菌株的选育及发酵工艺优化  

Breeding of High Epothilone B Producing Strain and Optimization of the Fermentation Conditions

作　　者：龚国利[1] 王娜[1] 刘丽丽[1] 

机构地区：[1]陕西科技大学生命科学与工程学院,陕西西安710021

出　　处：《食品与生物技术学报》2014年第11期1196-1203,共8页Journal of Food Science and Biotechnology

基　　金：国家自然科学基金项目(20906058);陕西省教育厅自然科学专项项目(12JK1023)

摘　　要：以纤维堆囊菌(Sorangium cellulosum)So F5-76为诱变出发菌株,经紫外(UV)和亚硝基胍(NTG)复合诱变和筛选,获得一株高产突变菌株So F5-H23,其发酵产埃博霉素B的量可达79.83 mg/L,比出发菌株提高了1.27倍。通过Plackett-Burman实验和响应面分析法对纤维堆囊菌产埃博霉素B的发酵工艺进行优化。得到最佳发酵工艺为:马铃薯淀粉4.8 g/L,葡萄糖0.5 g/L,脱脂奶粉2.3 g/L,豆饼粉2 g/L,七水硫酸镁2 g/L,无水氯化钙2 g/L,EDTA-Fe3+2 m L/L,微量元素(TE)0.5 m L/L,吸附树脂2%,培养基初始p H 7.4,装液量50 m L,接种体积分数8%,温度30℃。在此最优条件下埃博霉素B产量为108.67 mg/L,比优化前提高了36.13%,此产量是国内外报道的埃博霉素最高产量。A high epothilone B producing mutant SoF5-H23 was screened from Sorangium cellulosum SoF5-76 by combination treatment with UV and NTG.This mutant has good stability of producing epothilone B and production was 79.83 mg/L,which was 1.27 times higher than that of original strain.Plackett-Burman design and the response surface methodology （RSM） were applied to optimize medium for fermentation and conditions.The obtained optimal fermentation conditions were potato starch 4.8 g/L,skim milk powder 2.3 g/L,glucose 0.5 g/L,soybean powder 2 g/L,magnesium sulfate 2 g/L,chlorine calcium 2 g/L,EDTA-Fe3＋ 2 mL/L,trace elements 0.5 mL/L,absorbent resin 2％,pH 7.4,liquid volume 50 mL/250 mL,inoculum size 8％,temperature 30 ℃.Under this optimal conditions,epothilone B production reached up to 108.67 mg/L which is the highest yield of epothilone B on home and overseas and was 36.13％ higher than preliminary conditions.

关 键 词：纤维堆囊菌 埃博霉素B 菌株诱变 发酵工艺优化 响应面法 

分 类 号：Q936[生物学—微生物学]