HPLC法测定保健食品葛枳胶囊中葛根素的含量  

Determination of Puerarin in Health - Care Food of Gezhi Capsules by HPLC

作　　者：潘丽丽[1] 江帆[2] 

机构地区：[1]贵州工业职业技术学院,贵州贵阳550008 [2]贵州师范大学分析测试中心,贵州贵阳550001

出　　处：《贵州工业职业技术学院学报》2014年第4期25-28,共4页GUIZHOU SCIENCE AND TECHNOLOGY PROFESSIONAL COLLEGE

基　　金：贵阳市科学技术计划项目（[2009]筑科农8-004号）.

摘　　要：目的：建立葛枳胶囊中葛根素的含量测定方法。方法：采用高效液相色谱法，色谱柱Diamonsil C18（5μm，4．6mm×200mm），流动相为甲醇-36％乙酸-水=25：3：72，流速为0．8mL·min^-1，检测波长为250nm。结果：葛根素在0．06368～0．7005μg范围内线性关系良好，r=0．9999，平均回收率为103．39％，RSD为2．49％。结论：该方法专属性强，准确可靠，重复性好，可用于葛枳胶囊中葛根素的质量控制与分析。Objective： To establish the determination method of puerarin in Gezhi capsules. Methods： HPLC method was used. The chromatographic column was Diamonsil C18 （5μm, 4.6mm × 200 mm）. The mobile phase was methanol- 36% acetic acid- water （25：3：72）. The flow rate was 0.8mL·min^-1 and the detection wavelength was at 250nm. Results ： The linear of puerarin seas in the range of 0.063 68 -0.7005μg （r = 0. 9999）. The average recovery was 103.39% and RSD was 2.49%. Con- clusion： The method had a good specificity, and it was accurate, reliable and reproducible. It could be used for the quality control of puerarin in Gezhi capsules.

关 键 词：葛枳胶囊 葛根素 保健食品 高效液相色谱法含量测定 

分 类 号：O652[理学—分析化学]