音猬因子在乳腺癌中的表达及其临床意义  

Expression of sonic hedgehog in breast cancer and its clinical significance

作　　者：姚春[1] 邵力伟[1] 郑刚[1] 刘锦修[1] 郜朝霞[1] 任德发[1] 伍龙[2] 

机构地区：[1]江汉大学第二附属医院(武汉市第五医院)肿瘤外科,武汉430050 [2]武汉大学人民医院肿瘤科,430060

出　　处：《中华乳腺病杂志（电子版）》2014年第5期24-27,共4页Chinese Journal of Breast Disease(Electronic Edition)

基　　金：湖北省卫生厅科研基金资助项目(JX5B23);武汉市科技发展引导类计划项目--科技攻关项目(201161038348)

摘　　要：目的观察音猬因子(Shh)在乳腺癌组织中的表达及其临床意义。方法收集2008年1月至2012年12月在武汉市第五医院手术治疗的乳腺癌患者的乳腺癌组织和癌旁正常乳腺组织各46例。采用实时荧光定量PCR法和免疫组织化学染色检测乳腺癌组织及癌旁正常乳腺组织中Shh mRNA和Shh蛋白的表达,并探讨Shh蛋白与乳腺癌临床病理特征的关系。计数资料比较采用χ2检验,计量资料比较采用独立样本t检验或单因素方差分析。结果乳腺癌组织中Shh mRNA表达水平明显高于癌旁正常乳腺组织(1.54±0.47比0.60±0.38,t=-12.02,P=0.000),Shh蛋白阳性表达率也明显高于癌旁正常乳腺组织[69.6%(32/46)比17.4%(8/46),χ2=25.48,P=0.000],并且其表达水平与乳腺癌的临床分期密切相关,患者临床分期越晚,Shh蛋白表达水平越高[Ⅲ期+Ⅳ期∶Ⅰ期+Ⅱ期:77.8%(28/36)比4/10,χ2=5.28,P=0.022]。结论 Shh信号在乳腺癌组织中被激活。Shh异常表达可能与乳腺癌的发生、发展相关,其可以作为一个潜在的肿瘤标志物应用于临床。Objective To detect the expression of sonic hedgehog （Shh） in breast cancer tissue and its clinical significance. Methods Totally 46 patients with breast cancer who were treated by operation in Fifth Hospital of Wuhan City from January 2008 to December 2012 were enrolled. Their breast cancer tissues and normal para-carcinoma tissues were analyzed. The mRNA and Protein expressions of Shh in breast cancer tissues and normal paracarcinoma tissues were detected by real-time fluorescent quantitation PCR assay and immunohistochemistry and its relationship with clinicopathologic characteristics were also analyzed. Count data were compared using Chi-square test, and measurement data were compared with independent sample t test or One-Way ANOVA. Results The Shh mRNA level in breast cancer tissues was significantly higher than that in normal paracarcinoma tissues （ 1.54±0. 47 vs 0. 60±0. 38 ,t= -12. 02 ,P=0. 000）, and the positive rate of Shh protein in breast cancer tissues was significantly higher than that in normal paraeareinoma tissues [ 69. 6% （32/ 46 ） vs 17.4% （ 8/46 ） , χ^2 = 25.48, P = 0. 000 ]. Shh protein expression was closely related to elinicopathologic stage. The more advanced stage, the higher Shb protein expression rate [ stage Ⅲ ＋ Ⅳ ： stage Ⅰ ＋ Ⅱ = 77. 8% （28/36） vs 4/10, χ^2 =5.28,P= 0. 022 ]. Conclusions The Shh signal is activated in breast cancer tissues and its abnormal expression may play an important role in the occurrence and development of breast cancer. Shh may be used as a potential tumor marker in clinic.

关 键 词：乳腺肿瘤 猬蛋白质类 人类 病理学 临床 

分 类 号：R737.9[医药卫生—肿瘤]