豫北泥鳅线粒体DNA 16S rRNA和12S rRNA基因序列的遗传多样性分析  

Genetic Diversity of the mtDNA 16S rRNA and 12S rRNA Genes in the Population of Misgurnus anguillicaudatus from North Henan

作　　者：杜启艳[1] 董方娟 常重杰[1] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007

出　　处：《河南农业科学》2014年第11期137-140,146,共5页Journal of Henan Agricultural Sciences

基　　金：国家自然科学基金项目(30771666)

摘　　要：为了评价豫北地区泥鳅种质资源的多样性,以采自新乡地区泥鳅种群中的11个个体为材料,利用PCR技术扩增其线粒体16SrRNA和12SrRNA基因的部分序列,利用Clustalw 2.0和DNAsp 4.10软件分析了不同个体间的差异和遗传多样性。结果表明:16SrRNA和12SrRNA基因种内个体间的差异分别为0.25%和0.97%,二者的分化程度较低;16SrRNA的进化速度约为12SrRNA基因的4倍。16SrRNA和12SrRNA种群群体的单倍型间平均遗传距离分别为0.002 7、0.001 6,单倍型多样度指数分别为0.873、0.327,核苷酸多样性指数分别为0.002 65和0.000 81,平均核苷酸差异数分别为1.636和0.327。可见,豫北泥鳅种群具有较低的遗传多样性。In order to accurately evaluate the diversity of germplasm resources,to provide basic data and theoretical guidance for the genetic breeding of loach,the mtDNA 16S rRNA and 12S rRNA genes fragment of 1 1 individuals from the population of M.anguillicaudatus from the Xinxiang of Henan were amplified by PCR.The genetic diversity and sequence variation were studied via Using Clustalw 2.0 and DNAsp 4.10 software.These results indicated that the sequence variation among individuals were 0.9 7% and 0.2 5%.The genetic divergence was exceptionally low and the evolution rate of 16S rRNA was four times that of 12S rRNA.For the mtDNA 16S rRNA and 12S rRNA genes,the average genetic distance among the haplotypes in the population was 0.002 7 and 0.001 6,and the haplotype diversity was 0.873 and 0.327.The nucleotide diversity of the 11 individuals was 0.002 65 and 0.000 81,and the average number of nucleotide differences was 1.636 and 0.327.The results demonstrated that the genetic diversity of the population of M.anguillicaudatus from North Henan was very low and the protection for the population is necessary.

关 键 词：泥鳅 遗传多样性 16SRRNA基因 12SRRNA基因 

分 类 号：S917[农业科学—水产科学]