热激处理对甜柿外植体活性氧代谢的影响  

Effects of Heat Shock on Active Oxygen Metabolism of Diospyros kaki Thunb Explants

作　　者：杨谷良[1,2] 邓先珍[1,3] 范媛媛[1,2] 

机构地区：[1]经济林木种质改良与资源综合利用湖北省重点实验室,湖北黄冈438000 [2]黄冈师范学院生命科学学院,湖北黄冈438000 [3]湖北省林业科学研究院,武汉430075

出　　处：《湖北农业科学》2014年第19期4619-4621,共3页Hubei Agricultural Sciences

基　　金：国家十二五科技支撑计划项目(2013BAD14B05);湖北省自然科学基金项目(2006ABA186);黄冈师范学院校青年基金项目(09CQ087)

摘　　要：以宝华甜柿(Diospyros kaki c.v.‘Baohuatianshi’)为材料,在对试验材料进行热激处理后,检测了活性氧代谢相关生理指标的变化情况。结果表明,经过热激处理后,与对照相比,外植体内超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活力出现了提高,丙二醛(MDA)和过氧化氢(H2O2)的含量则有所降低。说明热激处理能够降低活性氧含量,保护了细胞膜结构的完整性,抑制了褐变发生。The leaf of ＂Baohuatianshi＂ was treated under 45 ℃ for 30 min and the parameters of active oxygen metabolismrelated physiological indeexes were investigated.Results showed that compared with the control,the activity of superoxide dismutase（SOD） and scavenger enzymes（CAT） could be improved in a short period after the heat shock treatment,and then the content of malondialdehyde（MDA） and hydrogen（H2O2） reduced.It is indicated that the content of active oxygen was reduced by the heat shock treatment,thus the cell membranes stability was protected and the heat shock treatment could effectively suppress tissue culture browning in persimmon.

关 键 词：甜柿(Diospyros kaki Thunb) 组织培养 热激处理 活性氧 保护酶 

分 类 号：S665.2[农业科学—果树学] Q945.79[农业科学—园艺学]