肝豆灵对Wilson病模型大鼠肝组织miRNA-122表达的影响  

Effect of Gandouling on miRNA-122 Expression in Liver Tissues in Rat Model of Wilson's Disease

作　　者：沈斌[1] 鲍远程[2] 蒋怀周[3] 张娟[2] 王艳昕[2] 方向[2] 

机构地区：[1]湖北中医药大学,湖北武汉430065 [2]安徽中医药大学第一附属医院脑病中心,安徽合肥230031 [3]安徽中医药大学中医临床学院,安徽合肥230038

出　　处：《安徽中医药大学学报》2014年第6期50-53,共4页Journal of Anhui University of Chinese Medicine

基　　金：国家自然科学基金项目(81202691)

摘　　要：目的研究肝豆灵对Wilson病(Wilsons disease,WD)大鼠肝组织miRNA-122表达的影响,探究其保护肝脏的机制。方法将24只Wistar大鼠随机分为空白对照组、模型组、青霉胺组和肝豆灵组。铜负荷喂养8周后,分别用青霉胺、肝豆灵以成人等效剂量灌胃,空白对照组及模型组均以等量生理盐水灌胃,每组均灌胃4周。观察各组大鼠肝功能及肝组织miRNA-122的表达水平。结果肝豆灵和青霉胺均可显著降低WD大鼠异常升高的血清丙氨酸氨基转移酶(alanine aminotransferase,ALT)、天冬氨酸氨基转移酶(aspartate aminotransferase,AST)、总胆红素(total bilirubin,TBil)(P<0.05,或P<0.01),显著升高WD大鼠肝组织异常降低的miRNA-122表达水平(P<0.01)。肝豆灵降低血清ALT、AST和TBil的作用及升高肝组织miRNA-122表达的作用显著优于青霉胺(P<0.05,或P<0.01)。结论肝豆灵保护WD大鼠肝功能的作用与其上调肝组织miRNA-122的表达有关。Objective To study the effect of Gandouling on the miRNA-122 expression in liver tissues of rats with Wilson＇s disease （WD） and to explore the action mechanism of Gandouling in liver protection.Methods Twenty-four Wistar rats were randomly divided into blank control group,model group,penicillamine group,and Gandouling group.All rats except those in the blank control group were given copper-overloaded diets for 8 weeks.Then,the penicillamine group and Gandouling group received penicillamine and Gandouling,respectively,by gavage at human-equivalent doses,while the blank control group and model group received an equal volume of normal saline by gavage; intragastric administration was performed for 4 weeks in each group.The liver function and hepatic miRNA-122 expression in each group were measured.Results Both Gandouling and penicillamine significantly reduced the levels of alanine aminotransferase （ALT）,aspartate aminotransferase （AST）,and total bilirubin （TBil） （P＜0.05 or P＜0.01）,which were abnormally elevated in rats with WD,and significantly increased miRNA-122 expression （P＜0.01）,which was significantly reduced in the liver tissues of rats with WD.Gandouling was significantly superior to penicillamine in reducing serum ALT,AST,and TBil levels and increasing hepatic miRNA-122 expression （P＜0.05 or P＜0.01）.Conclusion Gandouling can protect the liver function of rats with WD,possibly by increasing hepatic miRNA-122 expression.

关 键 词：肝豆状核变性 肝豆灵 青霉胺 miRNA-122 

分 类 号：R742.4[医药卫生—神经病学与精神病学] R285.5[医药卫生—临床医学]