不同配比微生物毒性传感器对河豚毒素毒性的测定及与HPLC法比较  

Biosensor Monitoring Tetrodotoxin with Toxicity Sensor of Different Ratio Microbes and Comparison with HPLC Methods

作　　者：陈婧[1] 杨卓[1] 

机构地区：[1]中国环境管理干部学院,河北秦皇岛066004

出　　处：《湖北农业科学》2014年第20期4952-4954,5037,共4页Hubei Agricultural Sciences

基　　金：河北省高等学校科学技术研究项目(Z2013161);中国环境管理干部学院科研课题(2014004)

摘　　要：河豚毒素传感器响应性能与固定化菌体的量和辅料的量都有一定关系。设计微生物量为10μL,不加辅料及加入1%、2%辅料3种微生物膜,以夹层法固定微生物膜,以氧电极为换能器,分别组装微生物传感器,通过测定传感器的线性范围、线性相关系数以及加标回收率等获知固定化膜的通透性的好坏和响应信号的大小,最后确定3种组合中较优膜为10μL菌悬液加入1%的辅料。以最优膜组装毒性传感器与高效液相色谱方法的对照发现两者的差异不显著,表明试验建立的毒性微生物传感器方法可以很好地测定河豚毒素的毒性。Tetrodotoxin sensor response performance is closely related with the amount of immobilized bacteria and accessories.Three kinds of microbial membrane were designed.The first one was 10 μL microbial without no accessory material.The second one was 10 μL microbial with 1％ accessory material.The third one was 10 pL microbial with 2％ accessory material immobilized by sandwich immobilization.A biosensor was fabricated based on oxygen electrode.By measuring the linear range,the linear correlation and the reclaim rate of the two sensor,the property of membrane and value of the response signal was found out.Results showed that 10 microbial with 1％ auxiliary materials was the optimal combination of the two membrane.The toxicity sensor was assembled with the optimal membrane and compared with HPLC method.The results showed that the difference between the two methods was not significant.The toxicity microbial sensor method could accurately measure TTX.

关 键 词：河豚毒素 毒性传感器 HPLC法 微生物膜 

分 类 号：S852.44[农业科学—基础兽医学]