Folin-Ciocalteu法测定大熊猫主食竹中多酚含量  

Determination of the Contents of Polyphenols in Fargesia denudata,the Main Food of Giant Penda by Folin-Ciocalteu Colorimetric Method

作　　者：赵莘[1] 段丽娟[1] 高健[1] 郑丽丽[1] 李俊清[1] 

机构地区：[1]北京林业大学省部共建森林培育与保护教育部重点实验室,北京100083

出　　处：《西北林学院学报》2014年第6期212-216,共5页Journal of Northwest Forestry University

基　　金：林业公益性行业科研专项(201404422)

摘　　要：为建立Folin-Ciocalteu法测定大熊猫主食竹缺苞箭竹（Fargesia denudata）总酚酸含量的方法。以没食子酸为对照品,研究了Folin-Ciocalteu法测定缺苞箭竹中酚酸类物质含量的适宜条件。结果表明,适宜的比色条件为Folin-Ciocalteu试剂与10%Na2CO3体积比为1∶6,Folin-Ciocalteu试剂3mL,显色温度25℃,反应时间2h,检测波长780nm。浓度为20-100μg·mL^-1时与其吸光值呈良好的线性关系,测定方法的平均回收率为99.4%。可见该法有较好的准确度和重现性。Aims：to establish a quantitative method of measuring total phenols in Fargesia denudata,the main food of giant panda.Methods：with gallic acid as reference substance to study the suitable conditions of the measurement by Folin-Ciocalteu method.Results：the optimum conditions for determining total polyphenols were as follows：the volume ratio of Folin-Ciocalteu reagent to 10% Na2CO3：1∶6,3mL of Folin-Ciocalteu reagent incubated at 25℃for 2h,followed by detection at 780 nm.Good linear relationship was found in the concentration of 20-100μg·mL^-1,the average recovery was 99.4%.

关 键 词：缺苞箭竹 FOLIN-CIOCALTEU法 总酚 

分 类 号：S795.2[农业科学—林木遗传育种]