消瘤平体外诱导人白血病HL-60细胞凋亡及机制研究  

Study on the apoptotic effect of xiaoliuping on the human leukemia HL-60 cells in vitro and its mechanism

作　　者：詹飞[1] 李灿[1] 李瑞生[1] 张玉洁[1] 

机构地区：[1]湖北文理学院附属襄阳市中心医院,湖北襄阳441021

出　　处：《实用药物与临床》2014年第12期1528-1531,共4页Practical Pharmacy and Clinical Remedies

摘　　要：目的研究消瘤平对人白血病细胞增殖的抑制及诱导凋亡的作用,揭示其抗白血病的部分作用机制。方法体外常规培养HL-60细胞,以不同浓度的消瘤平作用于细胞,MTT法检测细胞生长抑制率;流式细胞术检测凋亡率和细胞周期;ELISA法检测培养上清液中Caspase-3、Fas和Bcl-2的含量。结果与空白对照组相比,作用24 h后,消瘤平显著抑制HL-60细胞的增殖,且呈时间和剂量依赖性;提高HL-60细胞的凋亡率,并诱导出现G2-M细胞周期阻滞;消瘤平作用48 h后,Caspase-9和Fas蛋白表达量增加,Bcl-2表达减少。结论消瘤平抑制人白血病HL-60细胞的作用机制与阻滞细胞周期于G2-M期和诱导细胞凋亡有关,其诱导凋亡作用可能通过上调Caspase-9、Fas和下调Bcl-2而实现。Objective To investigate the inhibition effect of Xiaoliuping on the proliferation of human leukemia cells and the induction of apoptosis and reveal part of the anti-tumor mechanism.Methods Cells were cultivated in vitro,HL-60 cells in culture medium in vitro were given different concentrations of Xiaoliuping for 24 h.The inhibitory rate of the cells was measured by MTT assay,cell apoptosis and cell cycle was examined by flow cytometry.The expressions of Caspase-3,Fas and Bcl-2 in cultured supernatant were detected by ELISA assay.Results Xiaoliuping inhibited the growth of HL-60 cells in a dose-dependent and time-dependent manner.The cell apoptosis rate was elevated and G2-M phrase was arrested.Xiaoliuping significantly increased the expressions of Caspase-9 and Fas,decreased the expression of Bcl-2 after 48 h.Conclusion Xiaoliuping inhibits the proliferation of human leukemia HL-60 cells via the apoptosis induction and cell cycle inhibition,the effect of inducing apoptosis is achieved by increasing Fas and Caspase-9 and decreasing Bcl-2.

关 键 词：消瘤平 HL-60 增殖 凋亡 CASPASE-3 FAS Bcl-2 

分 类 号：R733.7[医药卫生—肿瘤]