基于KGdF_4:Dy^(3+)纳米材料检测土霉素的生物传感新方法  

A biosensor based on KGdF_4: Dy^(3+) nanoparticals for oxytetracycline determination

作　　者：吴世嘉[1] 聂雨[1] 张辉[2] 段诺[1] 王周平[1] 

机构地区：[1]江南大学食品学院食品科学与技术国家重点实验室,江苏无锡214122 [2]中国农村技术开发中心,北京100045

出　　处：《食品与机械》2014年第6期64-68,101,共6页Food and Machinery

基　　金：国家科技支撑计划项目(编号:2012BAK08B01);国家自然基金(编号:31401576)

摘　　要：土霉素是一种广谱抗菌剂,该研究对其建立一种快速灵敏的荧光共振能量转移生物传感分析方法。以KGd F4:Dy3+纳米粒子为荧光能量供体,氧化石墨烯为荧光能量受体,基于氧化石墨烯对单链DNA的π-π共轭作用,使得适配体功能化的KGd F4:Dy3+纳米粒子与氧化石墨烯足够靠近,从而发生荧光共振能量转移,KGd F4:Dy3+纳米粒子的荧光被淬灭。当体系中存在土霉素时,土霉素优先与其适配体特异结合形成复合物,与氧化石墨烯结合能力减弱,使得KGd F4:Dy3+纳米粒子的荧光淬灭程度降低,据此可实现对土霉素的定量检测。该方法在土霉素浓度1~120 ng/m L范围内与体系荧光强度呈现良好的线性关系(R2=0.992),最低检出限为0.59 ng/m L;且特异性好、灵敏度高,已成功应用于实际样品中土霉素的测定。We report on a method for oxytetracycline detection, which is a broad-spectrum antibacterial agent. It is based on fluorescence resonance energy transfer （FRET） from KGdF4 ：Dy3＋ nanoparticals as a donor, and on graphene oxide that acts as an acceptor. The KG- dF~ ：Dy3＋ nanoparticals were modified with oxytetracycline aptamer. On the basis of the strong π-πeffect between the nucleobases of the aptamer and graphene oxide, KGdF4： Dy3＋ nanoparticals were brought in close proximity to the graphene oxide surface. Therefore, the strong fluorescence of KGdF4 ：Dy3＋ nanoparticals can be signifi- cantly quenched. However, on addition of the target analyte, the ox ytetracycline-aptamer-KGdF4 ：Dy3＋ complex is formed and quench ing by graplaene oxide is suppressed. The fluorescence is linearly pro- portional to the concentration in the range from 1 to 120 ng/mI. （R2 ~ 0. 992）, with detection limits as low as 0.59 ng/mL. The as- say had high specificity and sensitivity, and was successfully applied to real food samples.

关 键 词：土霉素 KGD F4:Dy3+纳米粒子 氧化石墨烯 适配体 

分 类 号：TS207.3[轻工技术与工程—食品科学] TB383.1[轻工技术与工程—食品科学与工程]