丹参酮ⅡA抑制LPS诱导人单核细胞促炎性分子的表达  

Tanshinone ⅡA inhibits LPS-induced proinflammatory molecule expression in human monocytes

作　　者：王利月[1] 温洁霞[1] 林洪羽[2] 李文艳[1] 张建楼[1] 张永红[1] 李秀锦[2] 仲飞[1] 

机构地区：[1]河北农业大学动物医学院基础兽医系/河北兽医生物技术工程技术研究中心,河北保定071001 [2]燕山大学环境与化学工程学院生物工程系,河北秦皇岛066004

出　　处：《河北农业大学学报》2014年第6期85-90,共6页Journal of Hebei Agricultural University

基　　金：河北省自然科学基金(C2013204130);河北省教育厅重点科学研究项目(ZD20131056)

摘　　要：为探讨丹参酮ⅡA(Tanshinone IIA,Tan IIA)抗动脉粥样硬化(Atherosclerosis,AS)的影响,用脂多糖(Lipopolysaccharide,LPS)体外刺激人外周血单个核细胞(PBMCs)及人单核细胞系(THP-1),通过ELISA和RT-PCR方法分别检测细胞的促炎性细胞因子(IL-1β、TNFα、IL-6)和粘附分子VCAM-1的表达;同时用丹参酮IIA预先处理上述2种细胞,分析丹参酮IIA对LPS诱导细胞因子及粘附分子表达的影响。结果表明,LPS可明显诱导PBMCs和THP-1细胞IL-1β前体、TNFα、IL-6和VCAM-1的表达,而丹参酮ⅡA可明显抑制LPS诱导上述细胞因子及粘附分子表达,表明丹参酮ⅡA能够明显的抑制单核/巨噬细胞介导的炎性应答反应,为探索丹参酮ⅡA抗AS的机制提供了重要的试验依据。To illustrate the mechanism of Tanshinone Ⅱ A anti-atherosclerosis effect, the hu- man peripheral blood mononuclear cells （PBMCs） and human mononuclearcyte THP-1 were pretreated by Tanshinone Ⅱ A, and then primed by LPS. The proinflammatory cytokines （IL- 1β, TNFα and IL-6） and VCAM-1 adhesion molecule were measured by ELISA and RT-PCR. Results showed that LPS significantly induced the expression of IL-1β, TNFα, IL-6 and VCAM-1 in both PBMCs and THP-1 cells. Tanshinone Ⅱ A significantly inhibited the LPS-in-duced expressions of those cytokines and the adhesion molecule, indicating that Tanshinone Ⅱ A possesses the ability to inhibit monocyte-mediated inflammatory response. This study pro vides the important data for further investigations on Tanshinone Ⅱ A anti-atherosclerosis.

关 键 词：丹参酮ⅡA 人单核细胞系 单核/巨噬细胞 促炎性细胞分子 

分 类 号：R34[医药卫生—基础医学]