中药红曲对高脂血症大鼠PPARγ和脂联素受体表达的影响  

Effect of red kojic rice on expression of PPAR γ and adiponectin receptors in hyperlipidemic rats

作　　者：林希[1] 周毅[1] 徐苗苗[1] 林祥[1] 邵深深[1] 朱雪琼[1] 郎玮[2] 

机构地区：[1]温州医科大学温州市第三临床学院,温州市人民医院中医科,325000 [2]温州医科大学附属第一医院中医科

出　　处：《浙江医学》2014年第21期1767-1770,共4页Zhejiang Medical Journal

摘　　要：目的探讨中药红曲对高脂血症大鼠主动脉过氧化物酶体增殖物活化受体γ(PPARγ)和脂联素受体1和2(AdipoR1/AdipoR2)mRNA的影响。方法 SPF级雄性SD大鼠36只,随机数字表法分为正常组(12只)和高脂组(24只),高脂饲料喂养12周后高脂组造模成功,随机分为模型组和红曲组,每组12只,分别予0.9%氯化钠溶液和红曲水提液灌胃4周,检测血脂水平,HE染色观察主动脉病理改变,Western boltting检测PPARγ表达,ELISA检测血清脂联素水平,实时荧光定量PCR检测AdipoR1/AdipoR2 mRNA的表达。结果高脂组TG、TC和LDL-C升高,HDL-C下降(P<0.05);给药4周后,与模型组比较,红曲组TG、TC和LDL-C降低(P<0.01或0.05),HDL-C升高(P<0.05);红曲组血清脂联素、AdipoR1和AdipoR2 mRNA的表达较模型组升高(P<0.05或0.01)。结论红曲能降低高脂血症大鼠血脂水平,其机制可能与增加主动脉PPARγ蛋白的表达,升高血清脂联素水平及AdipoR1和AdipoR2 mRNA表达有关。Objective To investigate the effect of red kojic rice on expression of aorta peroxidase proliferator- activated receptorγ（PPARγ） and adiponectin receptor 1 and adiponectin receptor 2 （AdipoR1 and AdipoR2） mRNA in hyperlipidemic rat. Methods Thirty- six SPF male SD rats were randomly divided into control group （n=12） and hyperlipidemia group （n=24）. Hyper-lipidemia was induced by feeding high- fat diet for 12 weeks. The hyperlipidemic rats were further divided into model group and treatment group （n=12 in each group）, rats in two groups were given 0.9%normal saline and red kojic rice by gavage for 4 weeks, respectively. Serum lipid levels were measured and pathological changes in aorta were observed with HE staining. The expression of PPARγwas detected by Western blotting;serum adiponectin was measured by ELISA;AdipoR1 and AdipoR2 mRNA was de-tected by real time RT- PCR. Results After twelve weeks of high fat feeding, the triglyceride （TG）, total cholesterol （TC） and low density lipoprotein cholesterol （LDL- C） in hyperlipidemia group increased significantly （P〈0.01）, and high density lipoprotein cholesterol （HDL- C） decreased （P〈0.05）. Four weeks after administration, compared with the model group, the TG, TC and LDL- C in the treatment group were significantly decreased （P〈0.01 or 0.05）, while HDL- C increased （P〈0.05）. The aortic intimal thickening and the proliferation of smooth muscle cel s were decreased, the expression of PPARγand AdipoR1/AdipoR2 mRNA was increased in treatment group compared to model group. Conclusion Red kojic rice can lower the levels of blood lipids in hy-perlipidemic rats, which may be related to u aortic PPARγprotein expression and AdipoR1/AdipoR2 mRNA expression.

关 键 词：高脂血症 主动脉 红曲 过氧化物酶体增殖物活化受体Γ 脂联素 脂联素受体 PEROXIDASE proliferator- activated receptor γ 

分 类 号：R285.5[医药卫生—中药学]