老龄SHR大鼠胸主动脉PPARγ表达变化及罗格列酮干预作用  

Expression of PPARγ in the thoracic aorta of SHR with age and inhibitory effect of rosiglitazone

作　　者：袁海峰[1] 牛小麟[2,3] 高登峰[2] 郝光华 宋安琪 魏瑾[2] 

机构地区：[1]西安交通大学第二附属医院脑病科,西安710004 [2]西安交通大学第二附属医院心内科 [3]环境与疾病相关基因教育部重点实验室

出　　处：《陕西医学杂志》2014年第12期1571-1573,1578,共4页Shaanxi Medical Journal

基　　金：国家自然科学基金(No 81070219)

摘　　要：目的:探讨老龄SHR大鼠胸主动脉过氧化物酶体增殖物激活受体γ(PPARγ)表达变化情况及罗格列酮(Ros)干预作用。方法:64周龄Wistar-Kyoto(WKY)大鼠和自发性高血压大鼠(SHR)分为WKY,SHR及SHR+Ros组,每组9只。SHR+Ros组大鼠给予Ros(5mg/kg/d)灌胃,连续56d;WKY和SHR组大鼠分别灌服等体积的生理盐水。干预结束后采用tail cuff法测量各组大鼠尾动脉收缩压,采用HE染色观察主动脉组织病理学改变情况,采用免疫组化染色及图像分析检测胸主动脉PPARγ蛋白表达情况,采用real time-PCR检测主动脉PPARγmRNA表达情况。结果:与WKY大鼠相比,SHR大鼠血压升高(P<0.05),HE染色可见胸主动脉管壁增厚,内弹力板变直,间质增厚,细胞核着色较淡,免疫组化染色及real time-PCR结果表明SHR大鼠胸主动脉PPARγ蛋白及mRNA表达水平均下降(均P<0.05),与SHR组相比,SHR+Ros组大鼠血压降低(P<0.05),胸主动脉组织病理学改变情况缓解,管壁变薄,内弹力板趋于迂曲,间质变薄,细胞核着色变深,PPARγ蛋白及mRNA表达水平均增加(P<0.05)。结论:老龄SHR大鼠胸主动脉存在PPARγ表达下降,PPARγ配体罗格列酮可以逆转这种现象。Objectives：To investigate the expression of peroxisome proliferators-activated receptorγ（PPARγ）in the thoracic aorta of spontaneously hypertensive rats（SHR）with age and the inhibitory effect of rosiglitazone（Ros）on the expression of PPARγ.Methods：64-week old wistar-Kyoto rats（WKY）and SHR were randomly and respectively divided into WKY,SHR and SHR＋ Ros group,with 9rats in each group.The rats in SHR＋ Ros group were treated with Ros（5mg/kg,intragastrically）for 56 days,whereas normal saline was applied in WKY and SHR groups.Systolic blood pressure（SBP）of rats was measured by tail cuff method.Histopathological damage of thoracic aorta was analyzed by Hematoxylin-eosin（HE）staining.The levels of PPARγprotein and mRNA of thoracic aorta were detected by immunohistochemistry staining and real time-PCR respectively.Results：Compared with the rats in WKY group,the SBP of rats in SHR group was higher（P〈0.05）,and the levels of PPARγprotein and mRNA in thoracic aorta of rats in SHR group were lower respectively（P〈0.05）.In addition,HE staining revealed some remarkable histopathological abnormalities in thoracic aorta of rats in SHR group.The thickness of thoracic aorta was increased,and the elastic fiber was straight,and the interstitium between elastic fibers become thick,and the nucleuses were stained by hematoxylin slightly.Compared with the rats in SHR group,the SBP of rats in SHR＋Ros group was lower（P〈0.05）,and the histopathological abnormalities in thoracic aorta were attenuated.The thickness of thoracic aorta was decreased,and the elastic fiber was twisted,and the interstitium between elastic fibers become thin,and nucleus could be stained by hematoxylin deeply.Furthermore,the levels of PPARγprotein and mRNA in thoracic aorta of rats in SHR group were higher than those in SHR group respectively（P〈0.05）.Conclusion：The expression of PPARγin the thoracic aorta of SHR with age is decreased while thoracic aorta is aging,and rosiglitazone can play an inhibi

关 键 词：动脉 过氧化物酶体类 @罗格列酮 动物 实验 大鼠 

分 类 号：R364.1[医药卫生—病理学]