萝卜硫素对LNCaP细胞增殖、周期、凋亡及IGFBP-3、NF-κB表达的影响  

Effects of sulforaphane on proliferation,cell cycle and apoptosis of LNCa P and expressions of IGFBP-3 and NF-κB

作　　者：朱海鹏[1] 

机构地区：[1]郑州大学第五附属医院泌尿外科,郑州450052

出　　处：《郑州大学学报（医学版）》2014年第6期801-804,共4页Journal of Zhengzhou University(Medical Sciences)

摘　　要：目的:观察萝卜硫素(SFN)对人前列腺癌LNCa P细胞增殖、周期及凋亡的影响以及IGFBP-3及NF-κB在此过程中的变化。方法:观察不同浓度SFN对LNCa P细胞增殖的影响后将LNCa P细胞分为4组:对照组、SFN处理组、IGFBP-3 siRNA+SFN处理组以及IGFBP-3 siRNA处理组。MTT法分析各组细胞的增殖情况;流式细胞技术分析各组细胞的细胞周期及凋亡。实时荧光定量PCR技术和Western blot技术分析各组细胞IGFBP-3、NF-κB mRNA和蛋白表达的变化。结果:随SFN浓度的升高,LNCa P细胞存活率呈下降趋势(F=1 391.781,P<0.001)。IGFBP-3抑制能有效减弱SFN诱导的细胞增殖抑制、G2期抑制与凋亡(F交互=271.130、121.133和95.000,P<0.05)。IGFBP-3抑制可降低SFN处理后IGFBP-3 mRNA和蛋白表达的升高,升高SFN处理后NF-κB mRNA和蛋白表达的降低(F交互=8.595和279.490,P<0.05)。结论:SFN对LNCa P细胞的抑制作用可能与IGFBP-3有关,后者可能通过改变NF-κB的表达来发挥作用。Aim：To investigate the effects of sulforaphane on proliferation , cell cycle and apoptosis of LNCaP and ex-pressions of IGFBP-3 and NF-κB.Methods：The effects of SFN at different concentrations on LNCaP cells were observed . LNCaP cells were divided into four groups , including control group , 20 μmol/L SFN treated group , IGFBP-3 siRNA＋20μmol/L SFN treated group and IGFBP-3 siRNA treated group .MTT assay was used to evaluate the cell proliferation .Cell cycle arrest and the cell apoptosis were detected by flow cytometry .Further more, the expressions of IGFBP-3 and NF-κB in mRNA level and protein level were analyzed by real-time quantitative PCR and Western blot respectively .Results： SFN could inhibit the proliferation of LNCaP cells in a dose-dependent manner （F=1 391.781,P〈0.001）.The inhibition of IGFBP-3 could antagonize the proliferation inhibition , G2 phase arrest and apoptosis induced by SFN （Finteraction =271.130, 121.133 and 95.000, P〈0.05）.The inhibition of LNCap could depress the increased expression of IGFBP -3 mRNA and protein after SFN treatment , while increase the reduced expressions of NF-κB mRNA and protein by SFN treatment （Finteraction =8.595,279.490,P〈0.05）.Conclusion： SNF can inhibit the LNCaP cells′proliferation and the mechanism may associate with the changes of IGFBP-3, which may play a role in this process by changing the level of NF-κB.

关 键 词：萝卜硫素 前列腺癌 IGFBP-3 NF-ΚB LNCAP细胞 

分 类 号：R737.25[医药卫生—肿瘤]