杜仲叶水煎及醇提液中无机元素赋存形态分析  

Study on Combined Forms of Mineral Elements in both Water Decoction and Ethanol Extract of Eucommia ulmoides Olive's Leaves

作　　者：张琳杰[1] 张昌伟[1] 王志宏[1] 周云雷[1] 彭密军[1] 张水寒[2] 

机构地区：[1]吉首大学林产化工工程湖南省重点实验室,杜仲湖南省工程实验室,湖南张家界427000 [2]湖南省中医药研究院,湖南省中药新药研究与开发重点实验室,湖南长沙410013

出　　处：《分析科学学报》2014年第6期895-898,共4页Journal of Analytical Science

基　　金：湖南省教育厅高校产业化培育项目(No.11CY015);湖南省教育厅项目(No.13C758);吉首大学林产化工工程湖南省重点实验室开放项目(No.JDLC201208);吉首大学研究生科研创新基金(JGY201226)

摘　　要：通过水煎及醇提法对杜仲叶中铁、锌、铜、锰、钾、钠、钙和镁8种无机元素进行提取,分别用微孔滤膜、D101大孔树脂、732阳离子树脂、D402螯合树脂将无机元素分为初级形态及次级形态。采用环己烷-正丁醇分配体系模拟人体肠胃条件将无机元素分为脂溶态和水溶态,用火焰原子吸收光谱法（FAAS）对各种形态的无机元素进行测定。结果表明：杜仲叶醇提液中各无机元素提取率均高于水煎液;杜仲叶醇提液中8种无机元素的提取率为6.61%-89.30%;次级形态分析参数表明各无机元素均以某种次级形态为主的多种形态共存于可溶态溶液中。该法测定各元素的回收率为98.99%-104.0%,相对标准偏差在0.35%-1.11%之间,具有良好的精密度和准确度。The eight mineral elements Fe,Zn,Cu,Mn,K,Na,Ca and Mg in the leaves of Eucommia ulmoides Olive were extracted by means of water decoction and alcohol extraction.Millipore filter,D101 macroporous resin,732 cationic resin and D402 chelate resin were used to distinguish the different forms of mineral elements,i.e.,primary speciation and secondary speciation.The mineral elements were divided into lipid soluble and water soluble states by cyclohexane-n-butyl alcohol distribution system.The mineral elements of different speciation were determined by flame atomic absorption spectrometry（FAAS）.The results showed that extraction ratio of eight mineral elements by alcohol extraction were higher than water decoction.Analysis parameters of secondary speciation indicated that different forms of mineral elements coexisted in the solvable status and were given priority to one status.The recoveries of the elements were in the range of98.99%-104.0% with RSD in the range of 0.35%-1.11%,indieating good precision and accuracy.

关 键 词：杜仲叶 水煎液 醇提液 无机元素 形态分析 

分 类 号：O657.31[理学—分析化学]