基于因子分析方法筛选大鼠溃疡性结肠炎的敏感指标  

Screening of Sensitive Indexes of TNBS-induced Ulcerative Colitis in Rats by Factor Analysis

作　　者：赵益[1] 李冰涛[2] 刘燕[1] 赖小东[1] 刘红宁[1] 徐国良[1] 

机构地区：[1]江西中医药大学中医基础理论分化发展研究中心,江西南昌330034 [2]湖南中医药大学,湖南长沙410000

出　　处：《中药新药与临床药理》2014年第6期756-759,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家973计划课题(2010CB530603)

摘　　要：目的运用因子分析方法探讨三硝基苯磺酸(TNBS)+乙醇诱导的溃疡性结肠炎(UC)的敏感性指标。方法 SD大鼠随机分为正常对照组、模型组;以TNBS+乙醇诱导UC模型,模型复制5 d后测定大鼠体质量、隐血、稀便情况,计算疾病活动指数(DAI指数)和肠指数;比色法检测结肠组织匀浆上清液髓质过氧化酶(MPO)、丙二醛(MDA)、超氧化物岐化酶(SOD)、一氧化氮(NO)含量;酶联免疫吸附(ELISA)法检测血浆中白介素(IL)-4、IL-18、P-选择素含量。通过因子分析法评价各指标的主成分,并进一步计算出判别标准b值,以确定敏感指标。结果 UC在炎症和氧化方面指标经因子分析后,用主成分提取得到4个成分,特征指数1.382>1,判别标准b值的排序为DAI指数>肠指数>MPO>肠质量>MDA。结论利用因子分析可以指导UC大鼠模型敏感指标的筛选,可选取DAI指数、肠指数、MPO等作为大鼠UC敏感性指标。Objective To investigate the sensitive indexes of rat ulcerative colitis model induced by trinitro-benzene- sulfonic acid （TNBS） and ethanol-induced using the method of factor analysis. Methods SD rats were randomly divided into normal group and model group. Rat model of ulcerative colitis was induced by TNBS and ethanol. Five days after the modeling, we examined rat body weight, occult blood and loose stool, and calculated disease activity index （DAI） and colon index. The contents of medullary peroxidase （MPO）, malondialdehyde （MDA）, superoxide dismustase（SOD）, nitric oxide（NO） in colon tissue homogenate were detected by colorimetric method. The interleukin 4（IL-4）, IL-18, P-selectin levels of plasma were detected by enzyme-linked immunosorbent assay（ELISA）. Factor analysis method was used to evaluate the principal components of each index, and then the discriminant？criteria b value was calculated for defining the sensitive index. Results After factor analysis, 4 principal components of inflammation and oxidation were extracted, and the characteristic exponent was 1.382（ 〉 1 ）. The discriminant criteria b value for DAI, colon index, MPO, intestinal weight, MDA was in decreasing sequence. Conclusion The factor analysis method can be used for the screening of the sensitive indexes to evaluate rat ulcerative colitis induced by TNBS＋ethanol, and DAI, colon index, MPO, intestinal weight, MDA can be used as the sensitive indexes of UC rats.

关 键 词：因子分析 三硝基苯磺酸 溃疡性结肠炎 敏感指标 

分 类 号：R574.621[医药卫生—消化系统] R965.1[医药卫生—内科学]