基于454测序技术研究宁波沿海陆源排污口希瓦氏菌属的时空分布  

The research of space-time distribution for Shewanella in Ningbo land- based drainage outlets using 454 sequencing technology

作　　者：何伟娜[1] 王中华[1] 张迪骏[1] 童茜茜[1] 陈丽萍[1] 周君[1] 张春丹[1] 苏秀榕[1] 

机构地区：[1]宁波大学海洋学院,浙江宁波315211

出　　处：《海洋环境科学》2014年第6期896-901,共6页Marine Environmental Science

基　　金：海洋公益性行业专业专项资助项目(201105007);浙江省重大科技攻关项目(2006C13089);宁波市科技局资助项目(2008C50027)

摘　　要：基于454测序对宁波沿海10个陆源排污口在2011年的3月、5月、8月、10月四个月份的希瓦氏菌的分布进行了测定。结果表明:象山爵溪东塘排污口在3、8月份,象山西周工业园区综合排污口在5月份,距象山爵溪东塘排污口50 m以外的海域在5月份,象山墙头综合排污口、宁海颜公河入海排污口在8月份、象山石浦水产加工园区排污口在10月份均无希瓦氏菌属检出;而希瓦氏菌检出频数最高的是3月份的象山石浦水产加工园区工业排污口,出现频数为1 084次,其中已鉴定出的种有波罗的海希瓦氏菌Shewanella baltica(51)次,太平洋希瓦氏菌S.pacifica(46)次,希瓦氏菌ANA-3(1)次。从总体趋势来看,排污口处希瓦氏菌检出频数大于距排污口50 m以外的海域。The distribution of Shewanella based on 454 sequencing technology was determined in March,May,August and October,in 2011. The result shows that no detection of Shewanella in some stations,for example,the Xiangshan Juexi Dong Tang drainage outlet in March and August,Xiangshan Xizhou industrial comprehensive drainage outlet in May,the station 50 m from the Xiangshan Juexi Dongtang drainage outlet in May,Xiangshan Qiangtou comprehensive drainage outlet and Ninghai Yangong River drainage outlet in August,and Xiangshan Shipu aquatic products processing park drainage outlet in October. While the highest detection frequency of Shewanella is the Xiangshan Shipu aquatic product processing industry outlet in march（ 1084 times）,including Shewanella baltica（ 51 times）,S. pacifica（ 46 times） and S. ANA- 3（ 1 times）. According to the consequence,the detection frequency of Shewanella in drainage outlet is greater than the station 50 m away from drainage outlets.

关 键 词：宁波陆源排污口 454测序 希瓦氏菌属 时空分布 

分 类 号：X834[环境科学与工程—环境工程]