磺胺类抗生素与群体感应抑制剂对发光菌的联合毒性及其机制初探  

Joint effects and mechanisms of binary toxicity of sulfa antibiotics and quorum sensing inhibitors to Vibrio fischeri

作　　者：安情情 姚志峰[2] 顾宇菲 花文凤[3] 姜蕾[4] 林志芬[2] 印春生[1] 

机构地区：[1]上海海洋大学海洋学院,上海201306 [2]污染控制与资源化研究国家重点实验室,同济大学环境科学与工程学院,上海200092 [3]上海海洋大学水产与生命学院,上海201306 [4]城市水资源开发利用(南方)国家工程研究中心,上海200082

出　　处：《环境化学》2014年第12期2068-2075,共8页Environmental Chemistry

基　　金：同济大学污染控制与资源化研究国家重点实验室自主研究项目(PCRRY11003);国家自然科学面上基金(201177092;21377096);上海市教委重点学科(J50702);中央高校基本科研业务费专项资金(0400219181)资助

摘　　要：以Vibrio fischeri为模式生物,3类群体感应抑制剂(呋喃酮、吡咯酮和吡咯)与典型磺胺类抗生素为研究对象,测定了群体感应抑制剂与磺胺类抗生素联合急性毒性效应,通过群体感应抑制剂与信号分子竞争结合LuxR的实验和结合能Ebinding的测定,初步探讨了其联合毒性作用机制.结果显示,呋喃酮类化合物与磺胺类抗生素的二元等毒性比下的联合效应表现为协同与加和,吡咯酮类、吡咯类化合物与磺胺类抗生素的二元等毒性比下的联合效应分别表现为加和与拮抗;它们与LuxR的竞争结合力的差异,可能是它们与磺胺类抗生素等毒性比下的联合效应不同的原因.The acute joint effects of three quorum sensing inhibitors（ furanone, pyrrole and pyrrolone） and sulfa antibiotics to Vibrio fischeri were determined in this study. The mechanisms of joint toxic effects were explored by evaluating the competition between quorum sensing inhibitors and probe molecule for binding to LuxR and measuring the Ebinding. The result showed that three effects including synergic,additive and antagonistic effect were observed in experiment on binary toxicity tests. The reason for the different effects was proposed to be due to their different binding capacity with LuxR.

关 键 词：群体感应抑制剂 磺胺类抗生素 VIBRIO fischeri 联合急性毒性效应 

分 类 号：X171.5[环境科学与工程—环境科学]