高效液相色谱法测定鸟嘌呤的含量  

Determination of Guanine by HPLC

作　　者：俞建君[1] 陈文华[1] 

机构地区：[1]常州工程职业技术学院,江苏常州213164

出　　处：《广东化工》2014年第24期97-97,114,共2页Guangdong Chemical Industry

摘　　要：目的 建立用高效液相色谱法测定鸟嘌呤的含量.方法 采用Kromasil 100-5C18色谱柱;以1％乙酸-乙腈-甲醇（90-8-2）为流动相,柱温为25℃;检测波长273 nm;流速1.0 mL·min-1.结果 鸟嘌呤在（5～60） mg·L-1内线性关系良好（=0.9998）,最低检测限为0.0025 mg.L-1,平均回收率为98.1％.结论 本方法准确、灵敏,精密度高,可用于鸟嘌呤含量的测定.Objective To establish a high performance liquid chromatography method for the determination of the guanine. Methods The analytical column was Kromasil 100-5C18 and the mobile phase was 1% acetic acid-acetonitrile-methanol（90-8-2）,The coluran temperature was maintained at 25 ℃. The detection wavelength was 273 nm, the flow rate was 1.0 mL·min^-1. Results Guanine was linear in the range from 5 to 60 mg·L^-1 （r=0.9998）.The limit of detection was 0.0025 mg·L^-1. The average recoveries were 98. 1%. ConclusionO The method was proved to be accurate, sensitive and precise and showed highly specific. It can be applied for the content assay of guanine.

关 键 词：液相色谱法 鸟嘌呤 含量 

分 类 号：O65[理学—分析化学]