凋亡酶激活因子与p53及Ki-67在结直肠癌和结直肠腺瘤组织中的表达  

Expression of apoptotic protease activating facter-1,p53 and Ki-67 in colon cancer and colon adenma

作　　者：毕大明 董艳玲[2] 吴金朋[3] 王雪宁[3] 胡二斌 郑建国[1] 刘娜娜[1] 

机构地区：[1]河北省廊坊市第四人民医院病理室,廊坊065700 [2]河北省廊坊市第四人民医院口腔科,廊坊065700 [3]河北省廊坊市第四人民医院肿瘤科,廊坊065700

出　　处：《中国肿瘤临床与康复》2014年第12期1416-1419,共4页Chinese Journal of Clinical Oncology and Rehabilitation

摘　　要：目的探讨凋亡酶激活因子1(Apaf-1)、p53和Ki-67在结直肠腺瘤、结直肠癌组织中的表达情况。方法采用SP染色法,检测结直肠癌、腺瘤组织中Apaf-1、p53和Ki-67的表达情况。结果结直肠癌淋巴结转移组中,Apaf-1、p53、Ki-67阳性表达率分别为22.7%、31.8%和90.9%,无淋巴结转移组分别为43.6%、89.7%和61.5%,差异均有统计学意义(均P<0.05)。结直肠癌A、B期的Apaf-1、p53、Ki-67阳性表达率分别为43.6%、89.7%和61.5%,C、D期的Apaf-1、p53、Ki-67阳性表达率分别为27.7%、31.8%和90.9%。结直肠癌组织中Apaf-1阳性率(39.0%)低于腺瘤(88.0%),p53阳性率(77.0%)和Ki-67阳性率(68.0%)均高于腺瘤(分别为49.0%、26.0%),差异均有统计学意义(P<0.05)。Apaf-1阳性表达与p53、Ki-67阳性表达均呈负相关(r1=-0.358,r2=-0.361,P<0.01)。结论 Apaf-1、p53与Ki-67表达在结直肠癌、腺瘤的发生、发展与转移过程中有一定影响,对诊断结直肠癌有重要意义。Objective To explore the expression of apoptotic protease activating facter 1 （Apaf- 1 ）, Ki-67 and p53 in the human colon cancer and colon adenma. Methods The expressions of Apaf-1, Ki-67 and p53 were detected by immunohistochemistry in colon cancer and colon adenma. Results Tile positive expression rates of Apaf-I , Ki-67 and P-53 were 22.7% , 31.8% and 90. 9% in lymph node metastasis group respectively, which is different from those in non-lymph node metastasis group （43.6% , 89. 7% , 61.5% respectively）. The expression rates of Apaf-1, p53, Ki-67 were 43.6% , 89. 7%, 61.5% in colon tumor stages A and B respectively were different from those （22. 7% , 31.8% , 90. 9% respectively） in colon cancer stages C and D. The positive expression rate of Apaf-1 （39.0%） in tumor tissues were less than those of adenomas （88.0%） , the positive expression rates of p53 （77.0%） and Ki-67 （68.0%） were higher than those in adenoma （49. 0%, 26. 0% respectively）, with statistical significance （P 〈 0. 05）. The positive expression of p53 and Ki-67 had inverse correlation with Apaf-1 expression （r1 = -.0. 358, r2 = -0. 361, P 〈 0. 01 ）. Conclusion Apaf-1, p53 and Ki-67 play important roles in the process of the occurrence, development and metastasis of colon cancer and colon adenma, and has an important significance to instructs the diagnosis of colorectal cancer.

关 键 词：结直肠肿瘤 凋亡酶激活因子 P53 KI-67 

分 类 号：R735.3[医药卫生—肿瘤]